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山楂黄酮提取工艺研究
作者:张明
来源:《安徽农业科学》2014年第33期
摘要 [目的]优化山楂果实和叶中黄酮的提取工艺。[方法]采用单因素和正交试验法研究不同工艺条件对山楂黄酮提取效率的影响,得出最佳提取工艺及相关参数。[结果]试验得出,山楂黄酮提取采用最适宜方法为渗漉法,其最佳工艺参数是:溶剂为50%的乙醇,料液比1∶8 g/ml,浸泡时间72 h,流速为中速(3 ml/min)。 [结论]此方法工艺简单,成本低,提取率高,无需加热,剩余物无有害残留,适用于山楂黄酮的大批量提取生产。 关键词 山楂黄酮;黄酮提取;渗漉法;正交试验 中图分类号 S661.5 文献标识码
A 文章编号 0517-6611(2014)33-11857-02
Resrarch on extract technology to extraction of falconoid from hawthorn fruits ZHANG Ming (Chuxiong Medical College, Chuxiong, Yunnan 675005)
Abstract [Objective] To optimize technique for extracting flavonoidsfrom hawthom fruit and leaf. [Method] Using single-factor and orthogonal test method, effects of different technique conditions on extraction efficiency of flavonoids from hawthorn were studied. The optimum extraction technique and relevant parameters were obtained. [Result] The optimal method for extracting flavonoids from hawthorm is percolation. The best parameters are: 50% ethanol, solid-liquid ratio 1∶8 g/ml, soaking time 72 h, medium flow rate(3 ml/min). [Conclusion] This process is simple, low cost, high extraction rate, no need for heat, the residual is unharmful to be disposed. The technique is suitable for mass production of hawthorn flavonoid.
Key words Flavonoid of hawthorn; Extract flavonoids; Percolation method; Orthogonal design 基金项目 楚雄医药高等专科学校2013年校本项目。
作者简介 张明(1985- ),男,云南楚雄人,助教,硕士,从事天然产物提取、分离、纯化与生物活性测定研究。 收稿日期 2014-10-13
当今社会,高血压、高血脂等心脑血管疾病已严重影响人类的健康,因此,具有降压、降脂功效的药物研制一直受到人们的广泛重视[1]。