KitComponents:1mlDaily1kbDNALadder
Description:Daily1kbDNALadderisaproprietarymixtureofseveralplasmids
digestedtocompletionwithspecificrestrictionenzymestoproducealadderwith8fragmentsrangingfrom0.5-10.0Kb,producingthemosteconomicalladderinthe1KbDNAladderfamily.Theintensityofthe3Kband1Kbbandshasbeenincreasedtoyieldtwointernalreferenceindicators.6µlwillyieldatleastof30ngDNAinanysingleband.Storeat-20℃Storage:
Concentration:500ng/6µl
LoadingBufferComposition:
-10mMTris-HCl
-1mMEDTA(pH8.0)
-0.02%Bromophenolblue
-0.02%Xylenecyanol
-5%Glycerol
Usage:Addatleast6µlDaily1kbDNALadderdirectlytowellsdesignatedfor
markers.Youmayneedmorethan6µlofladder,dependingonwellsizeandlevelofintensityneededtovisualizethe
bands.