pFastBacHTC编号
北京华越洋VECT75431
pFastBacHTC载体基本信息：载体名称pFastBacHTC
启动子:复制子:终止子:质粒分类:质粒大小:质粒标签:原核抗性:筛选标记:克隆菌株:培养条件:表达宿主:诱导方式:5'测序引物:3'测序引物:pFastBacHTC载体图谱：
PC,polyhedrinpUCori，F1oriSV40poly(A)signal广宿主系列，昆虫细胞载体4858bpN-His；N-TEVAmpGenDH5α37℃，有氧LB昆虫细胞无须诱导，瞬时表达pFastbac-F:TATTCCGGATTATTCATACCpFastBac-R:ACAAATGTGGTATGGCTGApFastBacHTC载体简介：
ThepFastBacHTvectorissuppliedwiththemultiplecloningsitein
threereadingframes(A,B,andC)tofacilitatecloningyourgeneofinterestinframewiththeN-terminal6xHistag.pFastBacHTC载体序列：
ORIGIN1gacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgacc61gctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgcc121acgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgattt181agtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtggg241ccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagt301ggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgattta361taagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaattt421aacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaat481gtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatg541agacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaa601catttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcac661ccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttac721atcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgtttt781ccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgcc841gggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactca901ccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgcc961ataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaag1021gagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaa1081ccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatg1141gcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaa1201ttaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccg1261gctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcatt1321gcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagt1381caggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag1441cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcat1501ttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatccct1561taacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttct1621tgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctacca1681gcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttc1741agcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttc1801aagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgct1861gccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataag1921gcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacc1981tacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaaggg2041agaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggag2101cttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgactt2161gagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaac2221gcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcg2281ttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgc2341cgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatg2401cggtattttctccttacgcatctgtgcggtatttcacaccgcagaccagccgcgtaacct2461ggcaaaatcggttacggttgagtaataaatggatgccctgcgtaagcgggtgtgggcgga2521caataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaactag2581acagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttt2641tgttatggctaaagcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaaga2701ggggcgtggccaagggcatggtaaagactatattcgcggcgttgtgacaatttaccgaac2761aactccgcggccgggaagccgatctcggcttgaacgaattgttaggtggcggtacttggg2821tcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcg2881ggatcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctca2941tgcttgaggagattgatgagcgcggtggcaatgccctgcctccggtgctcgccggagact3001gcgagatcatagatatagatctcactacgcggctgctcaaacctgggcagaacgtaagcc3061gcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttacta3121cggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtct3181ccgaactcacgaccgaaaagatcaagagcagcccgcatggatttgacttggtcagggccg3241agcctacatgtgcgaatgatgcccatacttgagccacctaactttgttttagggcgactg3301ccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaaca3361tcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaa3421acagtcataacaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaa3481ggttctggaccagttgcgtgagcgcatacgctacttgcattacagtttacgaaccgaaca3541ggcttatgtcaactgggttcgtgccttcatccgtttccacggtgtgcgtcacccggcaac3601cttgggcagcagcgaagtcgaggcatttctgtcctggctggcgaacgagcgcaaggtttc3661ggtctccacgcatcgtcaggcattggcggccttgctgttcttctacggcaaggtgctgtg3721cacggatctgccctggcttcaggagatcggaagacctcggccgtcgcggcgcttgccggt3781ggtgctgaccccggatgaagtggttcgcatcctcggttttctggaaggcgagcatcgttt3841gttcgcccaggactctagctatagttctagtggttggctacgtatactccggaatattaa3901tagatcatggagataattaaaatgataaccatctcgcaaataaataagtattttactgtt3961ttcgtaacagttttgtaataaaaaaacctataaatattccggattattcataccgtccca4021ccatcgggcgcggatctcggtccgaaaccatgtcgtactaccatcaccatcaccatcacg4081attacgatatcccaacgaccgaaaacctgtattttcagggcgccatggggatccggaatt4141caaaggcctacgtcgacgagctcactagtcgcggccgctttcgaatctagagcctgcagt4201ctcgaggcatgcggtaccaagcttgtcgagaagtactagaggatcataatcagccatacc4261acatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaa4321cataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaa4381taaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgt4441ggtttgtccaaactcatcaatgtatcttatcatgtctggatctgatcactagatctgcct4501aggagatccgaaccagataagtgaaatctagttccaaactattttgtcatttttaatttt4561cgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataa4621tccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacagcgg4681ggcatttttcttcctgttatgtttttaatcaaacatcctgccaactccatgtgacaaacc4741gtcatcttcggctactttttctctgtcacagaatgaaaatttttctgtcatctcttcgtt4801attaatgtttgtaattgactgaatatcaacgcttatttgcagcctgaatggcgaatgg//pFastBacHTC载体其他相关的昆虫细胞载体：pFastBacHTCpFastBacHTBpFastBacHTApFastBacDualpFastBac1pAC5.1b-EGFPpVL1392pAcGP67A
pFastBac-GST-NpFastBac-GST-C