CACCGGTTAATTTCCCTTTGCTTTTGTG-3′,
GTGAAACTAATTTTCTTAATGATTTCTTTAGCGAC-3′;
psaA
(ATCG00350),
5′-5′-
ATGATTATTCGTTCGCCGGAACCAG-3′, 5′-GCTGCTTTGTGATAATGGAACCAACCA-3′; petA
5
(ATCG00540), 5′-ACCTTTTCTTGGATAAGGGAAGAGATTAC-3′, 5′-
ACATCTTTATTAGTAGCAGGGTCTGGAGC-3′; atpB (ATCG00480), 5′-TCCTACTAC TTCAAATCCA GAGGTTTCGA-3′, 5′-CACCAAATACGGATACACCACCATGAGCT-3′ DFR
(AJ251982),
5′-TTGAAGGTGTTGATGAGAATCTAAAGAGCA-3′,
5′-
ACCGTTACAATCACACGCGATACAAAATC-3′. For CHS (M20308), a 1.3-kb HindIII fragment of pCHS3.9 (Feinbaum and Ausubel, 1988) was used. For CAB (AY045673), a 0.5-10
kb BamHI/BglII fragment of the RAFL 08-09-P03 clone obtained from RIKEN BRC was used. At18S ribosomal RNA (X16077) 5′-GGTTGATCCTGCCAGTAGTCATATGC-3′ and 5′-GCCCTCCAATGGATCCTCGTTAAGG-3′. GCAGTCGACGATGAGGAACGAGAGGA-3′
Complementation
and
analysis:
(5′-5′-
GCACTGCAGGAAAACATCAGATGTTCACCT-3′). DNA gel blot analysis: 5′-15
GAGACCTGATTTCATAGTTATCTCAATAGG-3′ and 5′-
GTTACCGCAAATGCTATGTCACTGAG-3′. For AtTerC coding region of 35S::AtTerC:GFP: 5′-ACTAGTATGAGCTTAGCTTCAGTTATCCACCAC-3′ ACTAGTGCTGTCGCTGGATTTGTTTGTTAGG-3′
20
and 5′-
Barkan, A. (1998) Approaches to investigating nuclear genes that function in chloroplast biogenesis in land plants. Methods Enzymol. 297, 38-57.
Barneche, F., Winter, V., Crèvec?ur, M. and Rochaix, J.D. (2006) ATAB2 is a novel factor in the signalling pathway of light-controlled synthesis of photosystem proteins. EMBO J. 25, 5907-5918.
6
Feinbaum, R.L. and Ausubel, F.M. (1988) Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol. Cell. Biol. 8, 1985-1992.
Frick, G., Su, Q., Apel, K. and Armstrong, G.A. (2003) An Arabidopsis porB porC double mutant lacking light-dependent NADPH:protochlorophyllide oxidoreductases B and C is highly
5
chlorophyll-deficient and developmentally arrested. Plant J. 35, 141-153. Kim, J., Yi, H., Choi, G., Shin, B., Song, P.S. and Choi, G. (2003) Functional
characterization of phytochrome interacting factor 3 in phytochrome-mediated light signal transduction. Plant Cell, 15, 2399-2407.
Lichtenthaler, H.K. and Wellburn, A.R. (1983) Determination of total carotenoids and
10 chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 603, 591-592. Miras, S., Salvi, D., Ferro, M., Grunwald, D., Garin, J., Joyard, J. and Rolland, N. (2002) Non-canonical transit peptide for import into the chloroplast. J. Biol. Chem. 277, 47770-47778. Robinson, C. and Barnett, L.K. (1988) Isolation and characterization of chloroplasts. In CH Shaw, ed, Plant Molecular Biology: A Practical Approach. IRI Press, Oxford, pp 67-78.
15 Terry, M.J. and Kendrick, R.E. (1999) Feedback inhibition of chlorophyll synthesis in the phytochrome chromophore-deficient aurea and yellow-green-2 mutants of tomato. Plant Physiol. 119, 143-152.
7