the detection of MMP-9 antigen in the BM CD34+.
4. In our study, to establish the direct link between MMP-9 and CB CD34+ cells migration, we only determined the role of MMP-9 inspontaneous migration of CB CD34+ cells, but not in chemotactic migration. Actually, regulation of hematopoietic stem cell migration, homing and anchorage of repopulation cells to the bone marrow involves a complex interplay between adhesion molecules, chemokines, cytokines and proteolytic enzymes. Results obtained by the groups of Voermans reveal that not only the spontaneous migration but also the SDF-1 induced migration of CB CD34+ cells is greatly increased in comparison to CD34+ cells from BM and peripheral blood.
5. CD34+ cells we obtained in each cord blood sample were very limited. It is not enough to screen the inhibitors concentrations to select the optimal inhibitory concentrations. In the blocking experiments, based on the concentrations used by others and the manufacturer's recommendation, we then determined the inhibitors concentrations by excluding the toxicity of the inhibitors in that concentration, which was determined by clonogenic assay.
Minor comments:
1.The spelling and syntax errors have been checked and corrected.
2.Since the results in figure1Aand B were obtained from two separated and parallel experiments, it is not fitness to combine two figures.
这是我的一篇修稿回复，杂志是JBMR-A,影响因子3.652,已发表，供参考！
Reply to the comments on JBMR-A-05-0172
Comment:
Reference #10 is missing from the Introduction but used much later in the manuscript. Should these be in order used in manuscript? Reply:
The missing reference has been added into the revised manuscript.
Comment (continued):
What is the sample size for all tests performed?
Reply:
The sample size for drug release and PCL degradation tests was 3.0×3.0 cm2, with a thickness of about0.1mmand a weight of about 40mg. This dada have been added into the revised manuscript.
Comment (continued):
Figure 7. There is no scientific evidence presented in the TEM figure to convince this reviewer of sub-jets. This statement on Page 9 cannot be made without clear evidence during the jet formation/separation. Figure 7 is just a large fiber and small fiber fused together, no other conclusion than this can be made. Reply:
Necessary change in the statements has been made in the revised manuscript. as well as in the referred figure accordingly.
Comment (continued):
Table 3: Need standard deviation for all values reported not just for a select few.Equation after Table 3 not necessary. Just reference method used.
Reply:
Done accordingly.
Comment (continued):
Page 11: \analysis of this data? This reviewer does not see a significant difference in any of the data presented, thus weight loss would be considered equivalent.
Reply:
Although not too much difference was seen, the conclusion that “the GS/PCL membrane exhibited a relatively faster weight loss compared with the RT/PCL membrane” was indeed applicable through “one-way analysis of variance (ANOVA)” analysis. Following the reviewer’s comment, a new sub-section has been added to the manuscript. to address the statistical analysis for the data.
Comment (continued):
Page 12: What is the sample size for release data? Looks like results based on a sample size of one? Need stand deviations on the data presented in Figure 11. Why wasn't release
performed and compared for all electrospun conditions investigated otherwise?
Reply:
Three repeated tests were performed for each set of measurements and the resulting data were averaged. As stated in the revised manuscript, each sample had a square area of 33cm2 with a slightly different thickness.Standard deviations have been added to the data shown in Fig. 11.The present manuscript. aimed to show that medical drugs can be encapsulated in ultrafine fibers through a co-axial electrospinning process. The drug release data intended to show that the encapsulation was successful. We did not consider any specific application in this preliminary paper, and in fact the two drugs were just chosen as model illustration. As such, there seemed not necessary to perform. release experiments for all of the membranes electrospun with different conditions (i.e. the core concentrations)
Comment (continued):
Table 3: Yang's or Young's Modulus (page 10 says Young's).
Reply:
Corrected accordingly.
Comment (continued):
Figure 11: What is the % release, not just concentration. Why just this small sample of release data? Where is the release data for the other conditions?
Reply:
Unfortunately, we did not measure the amount of the shell material in obtaining the composite nanofibers. Namely, the flow rate of the shell solution during the electrospinning was not accurately controlled using an injecting pump. Hence the % release was not applicable. Please refer to the previous reply related to Page 12 and Figure 11 for the remaining comments.We acknowledge the reviewer’s comments and suggestions very much, which are valuable in improving the quality of our manuscript.
附3：SCI生物医学英文论文发表成功经验发表成功经验
SCI生物医学英文论文发表成功经验共享系列一---（Clinical Chemistry）
将自己近10年的科研工作中有关论文整理总结发表方面的一些信息贡献出来，与大家共享！如有时间，我拟将一些已经发表的文章，按照撰写与发表的实际经历与过程，即通过案例分
析每一个杂志的特色，审稿偏好，review意见及答复要点等逐一分析。可能包含的杂志系列有：nature methods，clinical chemistry，analytical chemistry，J. Clin. Immuno，Biomed. Microdev，Front. Biosci，Mol. Cell. Biochem，J. Expert，Rev. Proteomics，J biochemistry等。
本章先讲解美国Clinical Chemistry杂志，一个临床化学界的王牌杂志，近年其影响因子逐年攀升，现为7.7分。Clinical Chemistry由美国AACC每月出版，接受的文章包括与人体疾病相关的实验室研究，分析与分子诊断，仪器，资料处理，数据分析，临床研究等投稿。ISSN：0009-9147网络版ISSN：1530-8561
【URL】http://intl.clinchem.org/
【镜像URL】http://www.clinchem.org/
【出版者】American Association for Clinical Chemistry (AACC)
【收费情况】 免费,全文
【内容简介】
Clinical Chemistry is an international journal of laboratory medicine and molecular diagnostics.Clinical Chemistry -- This highly respected and often-cited scientific journal is published monthly and contains peer-reviewed methodology, research papers and other articles relevant to clinical chemistry and related laboratory sciences. Its circulation is more than 15,000.David E. Bruns, MD, Editor, (Charlottesville Office)
dbruns@clinchem.aacc.org
Sandra Weaver, Senior Editorial Assistant
sweaver@clinchem.aacc.org
Donna Brandl, Editorial Assistant
dbrandl@clinchem.aacc.org
Shane P. Cyr, Editorial Assistant
scyr@clinchem.aacc.org
Mac Fancher, Publisher, (WashingtonOffice)
mfancher@aacc.org
Miriam Gonzalez, Publications Coordinator
mgonzalez@aacc.org
【目录、摘要或全文上网等情况】
Free TOC, 1965 -
Free Abstract, 1975 -
Free Fulltext, 1997 -1999
Fulltext, 1997 -
【杂志被索引的情况】
Indexed in Chemical Abstracts.
【备注】
For faster access to Clinical Chemistry Online from these countries use this URL:
http://intl.clinchem.orgAustralia, Brazil, China, France, Germany, Hong Kong, Ireland, Israel, Italy, Japan, Mexico,Russia, Singapore, South Africa, South Korea, Spain, Sweden, Switzerland, Taiwan, The Netherlands, UK该杂志是由美国临床化学协会（American Association for Clinical Chemistry，AACC）主办的，于1948年成立，总部位于华盛顿，拥有1万余会员。先在网站注册，登记，按照提示一步步提供文章名称，摘要，作者姓名，所属领域，关键词，主文，图表等等。转换为PDF后就可以提交，然后给你一个查询号，接着就是等待了。。。
等了20多天，查阅状态看到了第一次回信：
Home Author Area Reviewer Area Personal Info. ClinChem Home Sign Out Submit New Manuscript. Information for Authors Queue Summary Feedback Help FAQ
Decision Letter
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