541 LSLLR
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COMPOSITIONAL ANALYSIS (extremes relative to: swp23s.q)
The composition of the input sequence is evaluated relative to the residue
usage quantile table specified with the `-s species' flag. Low usage in the 1% quantile is indicated by the label -- (e.g., Y-- means that the input sequence uses tyrosine as little as the 1% least tyrosine contain- ing proteins in the reference set); low usage in the 5% quantile is indi- cated by the label `-' (e.g., L-); high usage above the 95% quantile
point is indicated by the label `+' (e.g., A+); and high usage above the
99% quantile point is indicated by the label `++' (e.g., LIVFM++). The
usage is evaluated for all 20 amino acids, positive (KR) and negative (ED)
charge, total charge (KRED), net charge (KR-ED), major hydrophobics
(LVIFM), and the groupings ST, AGP (encoded by CCN, GCN, and GGN codons),
and FIKMNY (encoded by AAN, AUN, UAN, and UUN codons).
A : 65(11.9%); C : 2( 0.4%); D : 37( 6.8%); E : 20( 3.7%); F : 9( 1.7%)
G : 45( 8.3%); H- : 2( 0.4%); I : 30( 5.5%); K : 28( 5.1%); L : 51( 9.4%)
M : 6( 1.1%); N+ : 45( 8.3%); P- : 9( 1.7%); Q : 32( 5.9%); R : 15( 2.8%)
S : 43( 7.9%); T+ : 59(10.8%); V : 33( 6.1%); W : 0( 0.0%); Y : 14( 2.6%)
KR : 43 ( 7.9%); ED : 57 ( 10.5%); AGP : 119 ( 21.8%);
KRED : 100 ( 18.3%); KR-ED : -14 ( -2.6%); FIKMNY : 132 ( 24.2%);
LVIFM : 129 ( 23.7%); ST + : 102 ( 18.7%).
酶切结果预测
PeptideMass
The entered protein is: P06179
The selected enzyme is: Thermolysin
Maximum number of missed cleavages (MC): 0
All cysteines in reduced form.
Methionines have not been oxidized.
Displaying peptides with a mass bigger than 500 Dalton.
Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H]+.
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You have selected P06179 (P06179) from UniProtKB/Swiss-Prot:
Flagellin (Phase 1-I flagellin)
Chain Flagellin at positions 2 - 495 [Theoretical pI: 4.79 / Mw (average mass): 51480.50 / Mw (monoisotopic mass): 51450.00] mass position #MC modifications peptide sequence 1432.6590 236-249 0 VTGGTGKDGYYEVS 1238.5131 100-111 0 ANSTNSQSDLDS
1130.4783 310-319 0 MSYTDNNGKT
1109.4786 223-231 0 FDDTTGKYY
962.4789 250-258 0 VDKTNGEVT
844.4774 156-162 0 IDIDLKQ
840.4461 345-351 0 INTTKYT
830.4003 82-88 0 LNEINNN
820.3795 335-342 0 ATQNKDGS
818.3203 328-334 0 VGDDYYS
794.3526 352-359 0 ADDGTSKT
793.4566 175-180 0 VQQKYK
776.3784 275-281 0 ATEDVKN
770.3791 483-489 0 ANQVPQN
759.3995 120-125 0 LNEIDR
747.3883 287-293 0 ADLTEAK
741.2937 454-459 0 IEDSDY
733.3363 402-408 0 ATTTENP
719.3570 367-373 0 ADGKTEV
718.3730 210-216 0 LGGTDQK
717.3890 114-119 0 AEITQR
676.3624 18-23 0 LNKSQS
660.3675 422-427 0 LRSDLG
660.3562 217-222 0 IDGDLK
638.3508 376-381 0 IGGKTY
620.2886 460-465 0 ATEVSN
619.3046 126-131 0 VSGQTQ
606.2729 75-80 0 AQTTEG
606.2365 150-155 0 ANDGET
589.2940 13-17 0 LTQNN
562.2831 163-167 0 INSQT
557.3293 267-272 0 LTGGLP
549.2515 198-202 0 LDNST
548.2675 5-9 0 INTNS
548.2311 139-143 0 AQDNT
543.2773 393-397 0 AQPDL
527.2208 386-390 0 AEGHN
519.2409 41-45 0 AKDDA
516.3140 92-95 0 VREL
516.2889 429-432 0 VQNR
53.8% of sequence covered (you may modify the input parameters to display also peptides < 500 Da or > 100000000000 Da):
10 20 30 40 50 60
aqvINTNSl slLTQNNLNK SQSalgtaie rlssglrins AKDDAagqai anrftanikg 70 80 90 100 110 120 ltqasrnand gisiAQTTEG aLNEINNNlq rVRELavqsA NSTNSQSDLD SiqAEITQRL 130 140 150 160 170 180 NEIDRVSGQT QfngvkvlAQ DNTltiqvgA NDGETIDIDL KQINSQTlgl dtlnVQQKYK 190 200 210 220 230 240 vsdtaatvtg yadttiaLDN STfkasatgL GGTDQKIDGD LKFDDTTGKY YakvtVTGGT 250 260 270 280 290 300 GKDGYYEVSV DKTNGEVTla ggatspLTGG LPatATEDVK NvqvanADLT EAKaaltaag 310 320 330 340 350 360 vtgtasvvkM SYTDNNGKTi dgglavkVGD DYYSATQNKD GSisINTTKY TADDGTSKTa 370 380 390 400 410
420 lnklggADGK TEVvsIGGKT YaaskAEGHN fkAQPDLaea aATTTENPlq kidaalaqvd 430 440 450 460 470 480 tLRSDLGaVQ NRfnsaitnl gntvnnltsa rsrIEDSDYA TEVSNmsraq ilqqagtsvl 490 aqANQVPQNv lsllr
二级结构预测部分结果截图