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Alcoholic beverages are produced from a range of raw materials but especially from cereals, fruits and sugar crops. They include non-distilled beverages such as beers, wines, ciders, and sake. Disitlled beverages such as whisky and rum are produced from fermented cereals and molasses, respectively, while brandy is produced by distillation of wine. Other distilled beverages, such as vodka and gin, are produced from neutral spirits obtained by distillation of fermented molasses, grain, potato or whey. A variety of fortified wines are produced by addition of include sherries, port and Madeira wines.¾Æ¾«ÒûÁÏ¿ÉÓÉÐí¶àÔÁÏÀ´Éú²ú£¬µ«ÒÔ¹ÈÎˮ¹ûºÍÌÇÁÏ×÷ÎïΪÖ÷¡£¾ÆÀàÒûÁϰüÀ¨·ÇÕôÁóÒûÁÏ£¬ÈçÆ¡¾Æ¡¢ÆÏÌѾơ¢Æ»¹û¾ÆºÍÈÕ±¾Ã׾ơ£ÕôÁóÒûÁÏÈçÍþÊ¿¼ÉºÍÀÊÄ·¾Æ·Ö±ðÓɹÈÎïºÍÌǽ¬·¢½ÍÖÆµÃ£¬¶ø°×À¼µØÔòÊÇÓÉÆÏÌѾÆÕôÁóÖÆµÃ¡£ÆäËüÕôÁóÒûÁÏ£¬Ïó·üÌØ¼ÓºÍ¶ÅËÉ×Ó¾ÆÔòÓÉ·¢½ÍÌǽ¬¡¢¹ÈÎï¡¢ÂíÁåÊí»òÈéÇåËùµÃµÄ°×¾Æ¾ÕôÁóÖÆ³É¡£¸÷ÖÖ¸÷ÑùµÄÇ¿»¯ÆÏÌѾÆÊÇÔÚÆÏÌѾÆÖÐÌí¼ÓÕôÁóµÄ°×¾ÆÊ¹¾Æ¾«º¬Á¿Ìá¸ßµ½15£20£¥ÖƳɡ£ÖøÃûµÄ²úÆ·ÓÐÑ©Àæ°×ÆÏÌѾơ¢²¨¶û¶àºìÆÏÌѾƺÍÂíµÂÀÁÒÐÔÌð¾Æ¡£
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The yeast-cell envelope contains a plasma membrane, a periplasmic space and a cell wall consisting mainly of polysaccharides with a small amount of peptide material. The wall component is a semi-rigid but solute permeable structure which provides considerably compressional and tensile strength to yeasts. Carboxyl groups of cell-wall peptides confer important flocculating characteristics on brewing yeasts, facilitating post-fermentation solid-liquid separation. Flocculation is thought to result from salt- bridge formation between calcium ions and these cell-wall carboxyl groups. ½Íĸϸ°ûµÄ°üĤº¬ÓÐÒ»²ãÔÉúÖÊĤ£¬Ò»¸öÖÜÖʿռäºÍÒ»¸öϸ°û±Ú£¬Ï¸°û±ÚÖ÷ÒªÓɶà¾ÛÌǺÍÉÙÁ¿ëÄÎïÖÊ×é³É¡£±Ú×é·ÖÊǰë¸ÕÐÔµÄÈÜÖÊ͸ÐÔ¹¹Ô죬Ðí¶à×é°û±ÚµÄËõ°±Ëá»ùÍŸ³ÓèÄðÔì½ÍĸÒÔÖØÒªµÄÐõÄýÌØÐÔ£¬´Ù½øÁ˺󷢽͵ĹÌÒº·ÖÀë¡£ÐõÄý±»ÈÏΪÊÇÓɸÆÀë×ÓºÍÕâЩ°û±ÚôÈ»ù»ùÍżäÐγÉÑÎÇŵĽá¹û¡£
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The wort is inoculated with yeast to give to give a count of about 107 cells per ml wort or greater if a faster fermentation rate is required. traditionally, top-fermenting yeasts, which ferment at 15-22¡æ and tend to rise to the surface towards the end of fermentation and can therefore be skimmed off, were used in ale production. Yeasts, which ferment in the range 8-15¡æ and which sink to the bottom towards the end of fermentation , were generally used in lager production. However, particularly with the introduction of large cylindro-conical shaped fermenters and centrifuges to brewing operations, the distinction between top-and bottom-fermenting yeasts has tended to disappear.
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Freshly-fermented beer requires a variety of treatments before it is finally packaged for distribution. Maturation involves a secondary fermentation by the residual yeast which is transferred in the beer from the primary fermenter. During this process,beer diacetyl and small amounts of residual maltotriose are assimilated and concentration of some esters increases. Carbon dioxide, produced from the secondary fermentation, or sparged carbon dioxide, helps purge the beer of oxygen, h2s and unwanted volatiles. Additives for haze clarification, flavor aroma and colour adjustment, foam stability enhancement and microbiological stability are sometimes incorporated. The time and temperatures used vary from brewery to brewery. Usually low temperatures, 2-6¡æ, are used over a storage period ranging from 4 days to 4 weeks.
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After lagering the beer contains microbial cells, proteinaceous precipitates and colloidal matter, which are separated out by a variety of processes including flocculation, centrifugation and filtration. Microbiological stability is achieved by sterile filtration and /or by pasteurization.
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The flavour-enhancing properties of sodium glutamate were discovered in Japan at the start of the twentieth century and a fermentation process for its production by Corynebacterium glutamicum currently supplies and annual world market of about 400 000t.
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Even in the presence of excess biotin, glutamic acid producing bacteria, grown in
the presence of penicillin, can accumulate large amounts of glutamate. Penicillin inhibits bacterial cell-wall synthesis and the enhanced accumulation of glutamate is thought to result from the formation of swollen cells with weakened cell walls, resulting in damage to the permeability barrier of the cell membrane. ÔÚÇàÃ¹ËØ´æÔÚϵĹȰ±ËáÉú²ú¾ú£¬ÔÚ¹ýÁ¿ÉúÎïËØ´æÔÚµÄÌõ¼þÏÂÒ²ÄÜ»ýÀÛ´óÁ¿µÄ¹È°±ËáÑΡ£ÇàÃ¹ËØÒÖÖÆÏ¸¾úϸ°û±ÚµÄºÏ³É£¬µ¼ÖÂÁËÖ×ÕÍϸ°ûµÄÐγɣ¬ÏûÈõÁËϸ°û±Ú£¬ÆÆ»µÁËϸ°û ĤµÄÉøÍ¸Õϰ£¬Ìá¸ß¹È°±ËáÑεĻýÀÛ P75×îºóÒ»¶Î£º
Under optimized conditions for glutamate production from hexose, the Embden-Meyerhof-Parnas pathway predominates, channeling carbon precusors into the tricarboxylic acid. The NADPH + H+ formed in the oxidative decarboxylation(Ñõ»¯ÍÑôÈ) of isocitrate (ÒìÄûÃÊËáÑÎ) to ¦Á-ketoglutarate provides the reduced co-factor which together with NH3 is required for conversion of ¦Á-ketoglutarate to glutamateby glutamate dehydrogenase. Commercial glutamic acid-producing strains lack the tricarboxylic acid cycle enzyme ¦Á-ketoglutarate dehydrogenase and consequently, in the absence of NH4+ ions but with sufficient oxygen, ¦Á-ketoglutarate acid accumulates. Krebs cycle (¿ËÀײ¼Ë¹Ñ»·£¬¼´ÈýôÈËáÑ»·)intermediates, required for replenishment of oxaloacetate needed for the citrate synthase condensation reaction and other cell reactions, are supplied by efficient anaplerotic reaction and other cell reactions, are supplied by efficient anaplerotic reactions. Phosphoenolpyruvate carboxylase plays an important role in the carboxylation of phosphoenolpyruate to form oxaloacetate. Alternatively, the Krebs cycle intermediates may be replenished by the operation of the glyoxylate cycle. Stoichiometrically 1mole of glutamate is produced from 1.4 mole glucose when the glyoxylate cycle is involved whereas the pathway involving carbon dioxide fixation by producing 2 moles glutamate per mole of glucose. In order to increase the efficiency of conversion, some mutants have been introduced which have decreased levels of the glyoxylate cycle enzyme, isocitrate lyase. The metabolic pathway for production of glutamate from glucose is summarized in Fig.1.
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Yogurt is another well-known fermented dairy product that forms an important part of the human diet in many parts of the world. ËáÄÌÊÇÒ»ÖÖÖÚËùÖÜÖªµÄ·¢½ÍÈéÖÆÆ·£¬ÊÀ½çÉÏºÜ¶àµØ·½ÒѾ³ÉΪÈËÀàʳÎïÖØÒªµÄÒ» ²¿·Ö¡£
In the United States alone, sales figures indicated that more than 565 million pounds of this fermented dairy product were sold. 1979 ½öÔÚÃÀ¹ú 1979 Ä꣬ËáÄÌÏû·ÑÊý¾Ý±íÃ÷ÒѾ´ó´ó³¬¹ýÁË 565 °ÙÍò°õ¡£Depending on the system of manufacture and the nature of the coagulum, yogurts may be classified as being of two main types, set or stirred. ¸ù¾ÝÉú²ú¹¤ÒպͲúÆ·Äý¹ÌµÄÌØÐÔ£¬ËáÄÌ·ÖΪÄý¹ÌÐͺͽÁ°èÐÍ¡£ Tow lactose-fermenting bacteria , lactobacillus bulgaricus and streptococcus thermophilus , are used as the starter culture to make yogurt . 2ÖÖÊø×´ÈéÌÇ·¢½Í¾ú£º±£¼ÓÀûÑÇÈéËá¸Ë¾úºÍÊÈÈÈÁ´Çò¾ú±»ÓÃ×÷ËáÄÌÖÆ×÷µÄÅàÑø¾úÖÖ¡£Equal numbers of L.bulgaricus and S.thermophilus are desirable for flavor and texture production .µÈÁ¿µÄ±£¼ÓÀûÑÇÈéËá¸Ë¾úºÍÊÈÈÈÁ´Çò¾ú£¨Éú²ú³öµÄ£©²úÆ··çζºÍÖʸÐÊÇ×î¼ÑµÄThe lactobacilli grow first, liberating the amino acids glycine and histidine and stimulating the growth of streptococci.Èé¸Ë¾úÏÈÉú³¤£¬ÊͷŸʰ±ËáºÍ×é°±Ëᣬ²¢Äܹ»´Ì¼¤Á´Çò¾úµÄÉú³¤¡£The production of the characteristic flavor by the aforementioned cultures is a function of time as well as the sugar content of the starting yogurt material .ÓÉÉÏÊö¾úÖÖ²úÉúµÄ¶ÀÌØ·çζÊÇʱ¼äÓëËáÄÌ·¢½ÍÎïÖʺ¬ÌÇÁ¿µÄº¯Êý¡£ P89:
1.The resulting pressed grape juice ,called must,can be fermented naturally ,as is done in Europe ,by the enzymatic activity of wild yeasts that grow on the grapes.ѹեÆÏÌÑËùµÃµÄÖÒº£¬Ò²¾ÍÊÇÆÏÌÑÖ£¬¿ÉÒÔÀûÓÃÉú³¤ÔÚÆÏÌÑÉϵĽÍĸµÄøµÄ»îÐÔ½øÐÐ×ÔÈ»·¢½Í£¬ÏñÅ·ÖÞµÄ×ö·¨ÄÇÑù¡£
2.when the fermentation is compete ,as determined by the alcohol content of a sample ,the wine is placed in vats to classify and age.µ±·¢½ÍÍê³Éʱ£¬ÆÏÌѾÆ×°½øÍ°ÖнøÐгÎÇåºÍÀÏ»¯£¬·¢½ÍÊÇ·ñÍê³É£¬ÊÇÓÉÑùÆ·¾Æ¾«º¬Á¿¾ö¶¨µÄ¡£
3.Must from both red and white wine grapes is white and is used to produce white wine .Red wines are made by allowing the fermentation to proceed in the presence of red grape skins.ÆÏÌÑÖÀ´×ÔÓÚºìÆÏÌÑºÍ°×ÆÏÌÑ£¬°×ÆÏÌÑÖÓÃÀ´Éú²ú°×É«ÆÏÌѾơ£ºìÆÏÌѾÆÊÇÓÉ·¢½Í¹ý³ÌÖÐÔÊÐíÆÏÌÑÆ¤µÄ´æÔÚ¶øÀ´µÄ¡£
4.Certain wines are also distinguished from others on the basis of sweetness .In dry wines,for example, most or all of the sugar is Metabolized during fermentation.In sweet wine,fermentation is stopped while some sugar still remains in the product.ijЩÆÏÌѾÆÊÇ»ùÓÚÌð¶ÈÀ´Çø±ðÓڵģ¬ÔÚ¸ÉÆÏÌѾÆÖУ¬ÀýÈ磬´ó²¿·Ö»òÈ«²¿µÄÌÇÔÚ·¢½Í¹ý³ÌÖб»×ª»¯£¬ÔÚÌðÆÏÌѾÆÖУ¬µ±¾ÆÖл¹º¬ÓÐһЩÌǵÄʱºò·¢½Í¾ÍÍ£Ö¹ÁË¡£ 5.Since yeasts cannot survive in wines that are more than 15% alcohol,most table wines contain 10% to 12%.ÓÉÓÚ½ÍĸÔھƾ«º¬Á¿³¬¹ý15%µÄÆÏÌѾÆÖв»ÄÜ´æ»î£¬ËùÒÔ´ó¶àÊýµÄ¿ªÎ¸¾ÆµÄ¾Æ¾«º¬Á¿ÔÚ10%¡ª¡ª12%¡£
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Various techniques are used to determine the numbers and kinds of microorganisms and their products in foods. These methods include direct microscopic counts for both living and dead cells, standard plate counts, specific
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The concept of food safety has many facets. Perhaps the most obvious, and certainly the most prevalent problem of food safety is microbiological food poisoning. This is a serious problem in nearly every country, but another aspect of food safety is primarily concerned with chemicals. Some chemicals should be there and some should not. Chemicals in our food supply come from six sources. These are industrial accidents, intentional fraud, natural compounds, food additives, pesticides, and environmental pollutants. All require a different concept of food safety a different approach.
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Aflatoxin, a known carcinogen of high potency, is the product of a mold that occurs on peanuts and cereal grains, particularly in climates of high temperature and humidity. Herbal teas contain a number of potentially harmful substances; some of them, such as the alkaloid symphytine in comfrey tea, are carcinogens. Fortunately, none of these occur in amounts that would be harmful at normal levels of use¡£ »ÆÇúù¶¾ËØÊÇÒ»ÖÖ²úÉúÓÚ»¨ÉúºÍ¹ÈÎïµÄÖÚËùÖÜÖªµÄ¸ßÖ°©Îï£¬ÌØ±ðÊÇÔÚ¸ßκ͸ßʪÌõ¼þÏ£¨¸üÈÝÒײúÉú£©¡£ ²ÝÒ©²èÀຬÓÐÐí¶àDZÔÚµÄÓк¦ÎïÖÊ£¬ËüÃÇÖÐÈç³û¾Õ²èÖеÄÉúÎï¼îÎ÷·Ç¶¨£¨symphytine£©¾ÍÊÇÖ°©ÎïÖÊ¡£ÐÒÔ˵ÄÊÇ£¬ÔÚÕý³£µÄʹÓÃˮƽ£¬ËüÃÇÊDz»»á²úÉú¶¾º¦µÄ¡£
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Environment pollutants in land, air, and water can appear in food. Examples are lead from gasoline, cadmium from industrial metal plating, mercury in fish, from naturally occurring mercury in the ocean etc.
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