pBI121质粒图谱及载体信息
启动子：35S
终止子：Nos
% t+ f! b8 G% s报告基因：GUS
抗性基因：NptII
pBI121载体信息：
LOCUS AY781296 8196 bp DNA linear SYN 10-NOV-2004DEFINITION Expression vector pBI121_pro T-DNA region, complete sequence. ACCESSION AY781296
VERSION AY781296.1 GI:55418080 KEYWORDS .
1 R+ Y\; D$ h$ R9 @\- O, V2 p5 P: h7 c2 s+ G/ X, _2 X: K2 P% M4 G- w# R
SOURCE Expression vector pBI121_pro ORGANISM Expression vector pBI121_pro
other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8196)
AUTHORS Zeng,Y.H., Cao,J.W., Cai,H.Y., Chen,X.H. and Miao,L.X.
TITLE Construction of the plant expression vector of the proBA gene from Bacillus subtilis mutant to proline analog and analysis of the salt tolerance of transgenic Arabidopsis thaliana JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8196)
AUTHORS Zeng,Y.H., Cao,J.W., Cai,H.Y., Chen,X.H. and Miao,L.X. TITLE Direct Submission
JOURNAL Submitted (14-OCT-2004) College of Life Science, Wuhan University, Bayi Road, Wuhan, Hubei 430072, P.R.China FEATURES Location/Qualifiers source 1..8196
/organism=\ /mol_type=\ /db_xref=\ misc_feature 1..306
/note=\ gene 318..1112 /gene=\ CDS 318..1112 /gene=\ /codon_start=1 /transl_table=11
/product=\ /protein_id=\V51261.1\ /db_xref=\
/translation=\AQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF\ terminator 1113..1365 /note=\ promoter 2059..2859
/note=\ misc_feature 2872..3174
9 x5 Y0 z( o; l5 P7 z3 f! V4 Y- v t\3 x- N' }$ ?. d4 t7 T& g G2 v* b5 \\ ?& q x* X9 i1 J6 v* r* I! u7 _$ W$ O/ U6 D) a/ V\S% u+ T, T- }# b2 \\/ L0 a J6 v: M* W& P; t5 K3 ?! C% }7 U: Q3 X+ N5 \\& K, \\+ _/ _2 p# M\6 X$ d9 v\z/ ^! _8 \\ P6 l' c& o' o3 h; E1 C q9 z3 H& q- u3 Z- [- M4 B1 C8 l5 A8 B4 A/ K- d0 |4 a' u. n6 M9 y7 E$ L4 Z$ A\; R1 \\\`) D; F* l- B: I$ j+ D8 a4 F0 m' f$ ~; C- U3 * S7 s /note=\ Accession Number X86778\ gene 3175..4287 /gene=\ CDS 3175..4287 /gene=\ /codon_start=1 /transl_table=11
/product=\ /protein_id=\V51262.1\ /db_xref=\
/translation=\V
ILITSGAVAAGFSALGYPARPVTIKGKQAAAAVGQTLLMQQYMDHLKRYGLTPAQILL
TRNDFSKRERYRNAYATVMELLERGVVPIINENDSTSVEELTFGDNDMLSALVSGLIH
ADQLMILTDINGLYDANPNENPDAKRFDYLTDITPELLGYAGSAGSKVGTGGMKSKFL
AAQTALSLGVKVFIGTGAGREKLKVILDGKGDGTYIGDKELSTVNNTRQWIMFHSPIS
GEIIIDGGAEQAMIHNGSSLLPAGVADVCGSFPKGAVVEVRGPGGIIGKGQTHYSSDE
ILEAKGKRSDQLPGAKQVEVIHRNDWVNLFDEGENK\ misc_feature 4300..4602
/note=\ Accession Number X86778\ gene 4603..5733 /gene=\ CDS 4603..5733 /gene=\ /codon_start=1 /transl_table=11
/product=\ /protein_id=\V51263.1\ /db_xref=\
/translation=\ERQLI
LTENQKDIEAGQKRGLTPDIIDRLTLDEKRLLDIADAVELLIGLEDPVGESLETIQKE
NGLSIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSV
MKRALGLSKLPIDAVQLIEDTSKETAKQLFTLNDGLDVLIPRGGKNLIDLVVRESTVP
G! |2 ~: i/ r4 d) |3 L& q' F4 O1 V6 |, b, i+ Y: s/ |( z, v) S: r) F: Q\~$ S; |; O5 P+ {9 Q+ X) o- z, a6 e9 H3 h+ t& j* [( L5 l% I7 P\) X T+ p' V: }) i$ U, A# f( c4 } e7 P1 M- A6 {& f5 q& p* @% U0 [4 J\\& q: ~/ M* c5 K7 Y3 N2 j/ I& o\' Y9 Y# H7 {; W0 ^7 h; Y8 B. d% ?, a n* ~% U2 v# _( t6 ^\! j2 K# _3 o' x% N0 X( n! Q; _! M# \\& ~; J' Y( I9 q# ^5 D: @: l& C8 {1 P0 I( u: i# O6 E& z0 m9 ]& i! j7 R0 F( \\+ m# C VLETGAGNCHVYIDESADPDMARDVVINAKTQRPSVCNAIESLLIHEKWAEVHGRKLL
NQLTEKGVELRGDQMICELEPNAKQAEEADWGAEYLAPILSVKTVHDVQEAVRHIQQY
GTNHSEAILTENGEHAAYFQTAVDAAAVYHNASTRFTDGFEF\ gene 5758..7569 /gene=\ CDS 5758..7569 /gene=\ /codon_start=1 /transl_table=11
/product=\ /protein_id=\V51264.1\ /db_xref=\
/translation=\SRA
IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ\ terminator 7630..7882 /note=\ misc_feature 7883..8196
/note=\ ORIGIN
1 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta 61 agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact 121 gacagaaccg caacgattga aggagccact cagccgcggg tttctggagt ttaatgagct 181 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
\\2 g& W5 z8 W+ Y* C' }/ W# x! Y2 m- o6 t( Y5 W0 K* ^8 F H8 T3 {) j8 P' e4 ?( s8 l6 `4 g4 M* f0 o4 i: # \\* v1 5 g. & H7 f, P9 y* f8 h6 q9 c/ P/ R% l ^6 k3 S\S: 3 o. f! y A\8 g: kJ3 ], X! |3 F4 x; n\ U\. T0 Y- u4 l# l! c% ^% W& c( G, p+ }% [6 Z* A& r. |; d- q2 q7 m6 T3 s) y. P- {, K1 z5 ]7 _) V2 X' c: H7 D1 t6 ]& l' j9 w }! _; Q$ s, \\0 w9 V& L6 `3 a' [1 I& ~ 241 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc 301 aattaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 361 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 421 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 481 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 541 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 601 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 661 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 721 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 781 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 841 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga 901 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 961 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 1021 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 1081 ccttctatcg ccttcttgac gagttcttct gagatcgttc aaacatttgg caataaagtt 1141 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt 1201 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta 1261 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa 1321 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggcc tcctgtcaat 1381 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt 1441 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt 1501 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 1561 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 1621 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact 1681 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct 1741 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct 1801 tttgtctttg gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
1861 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 1921 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
1981 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 2041 aagcttgcat gcctgcagcc cacagatggt tagagaggct tacgcagcag gtctcatcaa 2101 gacgatctac ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga 2161 tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa 2221 gatcagaagt actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt 2281 aatagagatt ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa 2341 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag 2401 tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact 2461 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt 2521 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt 2581 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg 2641 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac 2701 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg 2761 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc 2821 ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactctag agtttgaaat
) b& h1 e% L% b0 N4 P7 n1 G7 ~, ]. {2 @' a% t- Y3 A3 M9 J: H# p\& P2 D\* F! N. y* r: y- C4 u, z0 {( y7 U: `3 & c; g# d* T1 L% z0 G,3 h% |% V! G B: U' @/ A* n$ E# q2 M& x$ X: K. e% z, A) [/ g2 D o) Z# A! v\* G5 _) y$ M% K1 C3 U# b# T, J! Z+ ]4 K+ v( ?6 d( t1 u4 d( I0 v6 e/ m8 {\* D- R9 I: }; v7 Y\1 X, X% C' O\ d- ^) \\( L, g; B, T7 c3 R$ f5 9 \\& w- v; i, ]1 z* A