BamHI EcoRI NheI 6Hisstop HindIII PT7 GST P3c cut site — MCS —
基因克隆表达功能区序列如下，经DNA双向测序验证 XbaI
tctagatcacacaggaaacagtattc atg tcc cct ata cta ggt tat tgg aaa att aag ggc ctt gtg caa ccc act cga ctt ctt ttg gaa tat ctt gaa gaa aaa tat gaa gag cat ttg tat gag cgc gat gaa ggt gat aaa tgg cga aac aaa aag ttt gaa ttg ggt ttg gag ttt ccc aat ctt cct tat tat att gat ggt gat gtt aaa tta aca cag tct atg gcc atc ata cgt tat ata gct gac aag cac aac atg ttg ggt ggt tgt cca aaa gag cgt gca gag att tca atg ctt gaa gga gcg gtt ttg gat att aga tac ggt gtt tcg aga att gca tat agt aaa gac ttt gaa act ctc aaa gtt gat ttt ctt agc aag cta cct gaa atg ctg aaa atg ttc gaa gat cgt tta tgt cat aaa aca tat tta aat ggt gat cat gta acc cat cct gac ttc atg ttg tat gac gct ctt gat gtt gtt tta tac atg gac cca atg tgc ctg gat gcg ttc cca aaa tta gtt tgt ttt aaa aaa cgt att gaa gct atc cca caa att gat aag tac ttg aaa tcc agc aag tat ata gca tgg cct ttg cag ggc tgg caa gcc acg ttt ggt ggt ggc gac cat cct cca aaa tcg gac agc atg cca gac tct ctc gaa gtt ctg ttt cag ggt cca gca gga tcc gca gaa ttc agc gct agc cac cat cat cat cat cat taa gctt HindIII
载体其它部分序列见pUC18(不含pUC18多克隆位点)
Glutathione S-transferase编码氨基酸序列如下（含多克隆位点）：
MSPILGYMKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDSMPDSLEVLFQGPAGSAEFSASHHHHHH 248 AMINO ACIDS MW 28.7KD
（六）pET-Trx vector size: 3.3kb 多克隆位点：
P3C cut site
PT7 RBS — TRX(his-patch —CTC GAA GTT CTG TTT CAG GGT CCA GCA GGA Leu Glu Val Leu Phe Gln Gly Pro BamHI TCC GCA GAA TTC AGC GCT AGC TAA CAT CAT CAT CAT TAA GCTT EcoRI NheI stop HindIII
BamHI EcoRI NheI stop HindIII PT7 p3c cut site — MCS —
基因克隆表达功能区序列如下，经DNA双向测序验证 XbaI
tctagaaataattttgtttaactttaagaaggagatatacatatgtctgataaaattattcatctgactgatgattcttttgatactgatgtacttaaggcagatggtgcaatcctggttgatttctgggcacactggtgcggtccgtgcaaaatgatcgctccgattctggatgaaatcgctgacgaatatcagggcaaactgaccgttgcaaaactgaacatcgatcacaacccgggcactgcgccgaaatatggcatccgtggtatcccgactctgctgctgttcaaaaacggtgaagtggcggcaaccaaagtgggtgcactgtctaaaggtcagttgaaagagttcctcgacgctaacctggctggctctagcatgccagactctctcgaagttctgtttcagggtccagcaggatccgcagaattcagcgctagctaacatcatcatcatcattaagctt HindIII 载体其它部分序列见pUC18(不含pUC18多克隆位点)
Thioredoxinmut(His-patch) (E.coli)编码氨基酸序列如下（含多克隆位点）：
MSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPILDEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGSSMPDSLEVLFQGPAGSAEFSAS 133 AMINO ACIDS MW 14KD
3 个His形成立体His-patch
（七）pET-His vector size: 2.9kb
多克隆位点： thrombin cut site
PT7 RBS — ATG His ?CTG GTG CCG CGC GGA TCC GCA GAA TTC AGC GCT Leu Val Pro Arg BamHI EcoRI AGC TAA CAT CAT CAT CAT CAT TAA GCTT NheI HindIII
BamHI EcoRI NheI stop HindIII
PT7 ATG-6His Thrombin cut site — MCS —
基因克隆表达功能区序列如下，经DNA双向测序验证 XbaI
tctagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggatccgcagaattcagcgctagctaacatcatcatcatcattaagctt HindIII
载体其它部分序列见pUC18 (不含pUC18多克隆位点)
(八)PTRC-CKS vector size: 5.2kb 多克隆位点：
P3C cut site
PTrc RBS — ATG—CKSmut —CTC GAA GTT CTG TTT CAG GGT CCA GCA GGA Leu Glu Val Leu Phe Gln Gly Pro BamHI TCC GCA GAA TTC AGC GCT AGC CAC CAT CAT CAT CAT CATTAA GCTT EcoRI NheI 6His-tag HindIII
BamHI EcoRI NheI 6His-stop HindIII
PTrc— RBS—ATG—CKSmut—p3C cut site—MCS
基因克隆表达功能区序列如下，经DNA双向测序验证 XbaI
tctagaaataattttgtttaactttaagaaggagatatacatatgagttttgtggtcattattcccgcgcgctacgcgtccacgcgtctgcccggtaaaccattggttgatattaacggcaaacccatgattgttcatgttcttgaacgcgcgcgtgaatcaggtgccgagcgcatcatcgtggcaaccgatcatgaggatgttgcccgcgccgttgaagccgctggcggtgaagtatgtatgacgcgcgccgatcatcagtcaggaacagaacgtctggcggaagttgtcgaaaaatgcgcattcagcgacgacacggtgatcgttaatgtgcagggtgatgaaccgatgatccctgcgacaatcatccgtcaggttgctgataacctcgctcagcgtcaggtgggtatggcgactctggcggtgccaatccacaatgcggaagaagcgtttaacccgaatgcggtgaaagtggttctcgacgctgaagggtatgcactgtacttctctcgcgccaccattccttgggatcgtgatcgttttgcagaaggccttgaaaccgttggcgataacttcctgcgtcatcttggtatttatggctaccgtgcaggctttatccgtcgttacgtcaactggcagccaagtccgttagaacacatcgaaacgttagagcagcttcgtgttctgtggtacggcgaaaaaatccatgttgctgttgctcaggaagttcctggcacaggtgtggatacccctgaggacggtagcatgccagactctctcgaagttctgtttcagggtccagcaggatccgcagaattcagcgctagccaccatcatcatcatcattaagctt HindIII
载体其它部分序列见pTrcHisB(不含pTrcHisB多克隆位点)
CKSmut(E.coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase or CMP-KDO synthetase)编码氨基酸序列如下(含多克隆位点)：
MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAERIIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEPMIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATIPWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIETLEQLRVLWYGEKIHVAVAQEVPGTGVDTPEDGSMPDSLEVLFQGPAGSAEFSASHHHHHH
268 Amino Acids Molecular Weight 29KD （九）PET-DsbA
vector size: 3.6kb
多克隆位点 thrombin cut site PT7 RBS — ATG-6His DsbA mut —ATG TAT CCG CGT GGA Met Tyr Pro Arg BamHI
TCC GCA GAA TTC AGC GCT AGC TAA CAT CAT CAT CAT CAT TAA GCTT 3? EcoRI NheI stop HindIII
BamHI EcoRI NheI stop HindIII PT7 RBS —ATG-6His DsbAmut Thrombin cut site—MCS
基因克隆表达功能区序列如下，经DNA双向测序验证） XbaI
Tctagaaataattttgtttaactttaagaaggagatataccatgggcagctctcatcatcatcatcatcactctagcggtgctcagtacgaagatggtaaacagtacactaccctggaaaaaccggtagctggcgcgccgcaagtgctggagtttttctcyyycttcagcccgcattcctatcagtttgaagaagttctgcatatttctgataatgtgaagaaaaaactgccggaaggcgtgaagatgactaaataccacgtcaacttcatgggtggtgacctgggcaaagatctgactcaggcatgggctgtggcgatggcgctgggcgtggaagacaaagtgactgttccgctgtttgaaggcgtacagaaaacccagaccattcgttctgcttctgatatccgcgatgtatttatcaacgcaggtattaaaggtgaagagtacgacgcggcgtggaacagcttcgtggtgaaatctctggtcgctcagcaggaaaaagctgcagctgacgtgcaattgcgtggcgttccggcgatgtttgttaacggtaaatatcagctgaatccgcagggtatggataccagcaatatggatgtttttgttcagcagtatgctgatacagtgaaatatctgtccgagaaaaaaggtgatgaagatgaagatgaagatagcatgtatccgcgtggatccgcagaattcagcgctagctaacatcatcatcatcattaagctt HindIII 载体其它部分序列见pUC18(不含pUC18多克隆位点)
DsbAmut(E.coli)编码氨基酸序列如下(含多克隆位点)：
MGSSHHHHHHSSGAQYEDGKQYTTLEKPVAGAPQV;EFFSFFSPHSYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALGVEDKVTVPLFEGVQKTQTI RSAS DIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKAAADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKKGDEDEDEDSMYPRGSAEFSAS 223 Amino Acids
Molecular Weight 24.7KD