TruSeq?SmallRNALibraryPrepGuide
FORRESEARCHUSEONLY
RevisionHistoryIntroduction
AdditionalResources
RNAInputRecommendationsLibraryPrepWorkflowPrepareAdapterSetupLigateAdapters
ReverseTranscribeandAmplifyPurifycDNAConstructValidateLibraryNormalizeLibrariesSupportingInformationTechnicalAssistance
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ILLUMINAPROPRIETARYPart#15004197Rev.G
Catalog#RS-200-9002DOCDecember2014
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RevisionHistory
Part?#15004197RevisionGDateDecember2014DescriptionofChange?Kitnamechangedfrom'sample'prepto'library'prep?SpecifiedminimumamountifpurifiedsmallRNAisusedasinput?ReplacedWhat'sNewsectionwithRevisionHistory?Updatedworkflowdiagramtoincludeconsumables?RenamedLigate3'and5'AdapterstoLigateAdapterswithnochangetotheprocess?RenamedDNA?TemplateStoragetoNormalizeLibrarieswithnochangetotheprocess?ModifiednameofNovexTBEconsumablestomatchmanufacturername?UpdatedRunGelElectrophoresisprocedurestoindicatemixingvessel?UpdatedAdditionalResources?UpdatedSDSlinktosupport.illumina.com/sds.html?CreatednewAdditionalResourcessection.?ReplacedTrackingToolsandBestPracticeswithareferencetocontentontheIlluminawebsite.?AddedreferencetoBaseSpace?toorganizesamples,libraries,pools,andruns.?CreatednewsectionofSupportingInformationcontainingthefollowingcontent:?Acronyms?KitContents?ConsumablesandEquipment?IndexedAdapterSequences15004197FFebruary2014Part?#RevisionDate15004197EFebruary2013DescriptionofChange?Movedworkflowdiagramtostartofprotocol?Reformattedtheconsumableslistatthestartofeachproceduretoincludeitemquantityandstoragetemperature?CorrectedtotalvolumeofRNAandRTPmixinPerformReverseTranscriptionprocedure?CorrectedannotationsonlowerimageofSmallRNALibraryfromTotalRNASamples(Figure12)?Changedfinallibrarystorageconcentrationto2?nMPart?#15004197RevisionDDateMay201215004197CApril2011DescriptionofChange?AddedaWhat'sNewsection?CreatedaGettingStartedsectiontocontainthefollowingsections:?AddedAcronyms?BestPractices-containsLiquidHandling,AvoidingCross-Contamination,TemperatureConsideration,andEquipmentinformation?RNAInputRecommendations?TrackingTools:?Reviseddocumentationdownloadinformation?Removedsamplesheetformatguidelinesanddirectreadertosequencinganalysissoftwareuserguidefordetailedsamplesheetguidelines?IntroducedIlluminaExperimentManager?IndexedAdapterSequences(renamedfromIndexes)?Pooling?KitContents?ConsumablesandEquipment:?Reviseduser-suppliedT4RNALigase2,DeletionMutantconsumableandsupplier?RemovedtextindicatingthataClusterGenerationKitisonlyrequiredwhennotperformingethanolprecipitation?RevisedtheLigate3'and5'AdaptersprocedurestospecifytheuseofT4RNALigase2,DeletionMutant.?Specifiedusingapreheatedlidinthermalcyclerpreparationandprocedures?RemovedthepreparationsectionfromeachprocedureintheExperiencedUserCardandincorporatedpreheatinginstructionsasstepsintheaffectedprocedures.?UpdatedSampleSheetsectiondescribinghowtohandlelaneswithasinglesample?Updatedprotocolamplificationandpurification?Updatedkitcontentsandusersuppliedconsumables