甘肃农业大学生命科学技术学院 发育生物学课程论文
样的现象。在拟南芥悬浮培养物中，ABA不能诱导NO的合成（Tun et al.,2001），说明ABA诱导的NO合成具有组织特异性。在豌豆中，ABA诱导的保卫细胞NO的合成是气孔关闭所需要的，当用清除剂PTIO去除NO时，ABA诱导的气孔关闭则被显著抑制。药理学证据表明NOS作为一种NO的来源，ABA诱导的气孔关闭和NO的合成都被L-NAME抑制，但不被钨酸盐抑制（Neill et al.,2002a）。
NO对植物的其他作用也可能与激素有某些关系，例如玉米根尖的生长既可被生长素促进，也可被多种释放NO的药品处理所刺激。Pagnussat等（2002）报道生长素能够诱导黄瓜根合成NO，虽然NO的合成途径还没有确定，但黄瓜根的生长和侧根的形成需要NO。NO的作用位点应处于生长素的上游，因为NO清除剂不能抑制生长素的作用（Gouvea et al.,1997），但目前仍然不知道NO是否参与生长素合成。现已证明植物的种子萌发、下胚轴生长、叶片生长及脱黄化等光敏色素调节的光控发育过程也涉及NO的作用（Beligni and Lamattin,2000; Giba et al.,1998;Leshem and Haramaty,1996），深入研究这些过程中的NO与各种植物激素的关系将有助于阐明NO在植物中的作用方式。
4. NO的信号转导
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甘肃农业大学生命科学技术学院 发育生物学课程论文
SA的生物合成 MAPKs NO 与含有铁的蛋白质相互作用，调节其活性 磷酸蛋白激酶的活化 蛋白质巯基的 S-亚硝酸化 GCs的活化 cGMP 被(cGMP)-PDEs代谢 cGMP-门离子通道的活化 cADP 代谢？ 通过从胞内贮藏库释放，细胞质[Ca2+] 已确定的途径 可能的途径 图2 NO的信号转导
Fig.2 NO signaling transduction
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甘肃农业大学生命科学技术学院 发育生物学课程论文
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