RESEARCHLETTER
ExtendedDataFigure9|Long-termBRL37344treatmentinhibitsJAK2(V617F)1HSCexpansionbutitdoesnotcompromisenormal
haematopoiesis.a,b,HaematopoieticprogenitorsquantitatedbyFACS(a)orcolony-formingunits(CFU-C)(b)inBMandbloodofWTmice12-16weeksaftertransplantationwithMPN(a)andWTBMcells(b),16weeksafterBRL37344(2mgkg21)orvehicleinjections(twiceaday,i.p.;n53).
Mean6s.e.m.*P,0.05(unpairedtwo-tailedttest).c,BRL37344treatmentdoesnotchangenormalHSCBMnumber.CD45.2WTmiceweretransplantedwithBMcellsfromcongenicCD45.1WTmice,togetherwithlimitingnumbersofBMcellsfromCD45.2controlmicetreatedwithvehicle/BRL37344over4weeks,starting11weeksafterthetransplant(n55).Thefrequencyofmicethatfailedreconstitution16weeksaftertransplantationisplottedagainstthenumberoftestedcells.HSCfrequenciesareindicated;n.s.,non-significant;Pearsonchi-squaredttest.
?2014Macmillan Publishers Limited. All rights reservedLETTERRESEARCH
ExtendedDataFigure10|DifferentiallyexpressedgenesinBM
CD452CD312Ter1192GFP1cellsfromNes-gfp;Mx1-cre;JAK2(V617F)mice.a,b,Topmostupregulated(a)anddownregulated(b)genesinRNaseqanalysesofBMCD452CD312Ter1192GFP1cellsfrom
Nes-gfp;Mx1-cre;JAK2(V617F)mice(red)versuscontrolmice(blue).Plotsrepresentdifferentiallyexpressedgenesinfragmentsperkilobaseofexonpermillionfragmentsmapped(FPKM).
?2014Macmillan Publishers Limited. All rights reserved