图8. A.HOTTIP在人不同组织器官中的表达情况B. E13.5小鼠胚胎上HOTTIP的原位杂交 2. 通过RNAi技术可以下调lncRNA HOTTIP的表达量
图9. A. 针对HOTTIP设计的siRNA可以下调HOTTIP基因及距离40 kb左右的编码基因HOXA13、HOXA11、HOXA10、HOXA9和HOXA7的表达量。B.对HOTTIP基因的干扰不影响HOXD基因和BID基因的表达
3. 动物实验验证HOTTIP干扰载体的表达可以影响HoxA基因的表达及骨质的形成。
图10. A. FISH结果显示HOTTIP干扰载体在鸡胚胎中的过表达影响HoxA基因的表达。B. HOTTIP基因表达量的下降导致远端骨头变短。
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