病害防治
第1期 朱天辉等:枯草芽孢杆菌菌种退化及其控制35
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AStudyonStrainDegenerationandItsControl
ofBacillussubtilis
ZHUTian2hui1,YANGZuo2zhong2
(1.FacultyofForestandHorticulture,SichuanAgriculturalUniversity,YaπanSichuan625014,China;2.SichuanGeneralStationforControllingForestDiseaseandInsect,ChengduSichuan610081,China)
Abstract:Thedegenerationof2strain(BS-3andPRS5)invariousmediaandsubculturingwasstudiedwiththedecreaseofantagonismandbiomassofBacillussubtilisasthedegenerationπstoken,andtheneffectsof5carriersandrejuvenescerttimeontherestorationofthebiomassandantagonismforB.subtiliswerealsoexplored.Ofthesevenregularmedia,B.subtilismoststablygrewonSDAY,butthosenutritionpooronessuchasPDA,WPD,CMAcauseddegenerationfrequently,thedegenerationπsrateofBS-3wasslowerthanthatofPRS5.Among5rejuvenescentcarriers,thecarrierwhichcontainedsteriledsoil,1%peptone,1%glucoseand0.5%yeastabstractwasregardedasthebestone.Inrejuvenescentrate,PRS5was90daysand120daysfasterthanBS-3respectively.
Keywords:Biologicalcontrol;Bacillussubtilis;Rhizoctoniasolani;Penicilliumspp;Fruit