红参和西洋参人参皂苷含量测定中的分析比较
图1 对照品(A、C、E)和红参样品(B、D、F)图谱Fig1
Chromatogramsofreferencesubstances(A,C,E)andRadixetRhizomaGinsengRubrasample(B,D,F)
A、B.HPLC(DiamonsilC18) C、D.HPLC(MerckC18) E、F.RRLC(AgilentZorbaxEclipseXDB-C18)1.人参皂苷Rg1(ginsenosideRg1) 2.人参皂苷Re(ginsenosideRe) 3.人参皂苷Rb1(ginsenosideRb1)
表1 系统适用性比较
Tab1 Comparisonofsystemsuitability
tR/min
方法(method)
色谱柱(column)
人参皂苷Rg1(ginsenosideRg1)
HPLC
DiamonsilC18MerckC18
RRLC
AgilentZorbaxEclipseXDB-C18
47 114 97 5
人参皂苷Re(ginsenosideRe)
48 115 98 0
人参皂苷Rb1(ginsenosideRb1)
79 926 215 7
分离度(resolution)
人参皂苷Rg1与人参皂苷Re(ginsenosideRg1andginsenosideRe)
1 551 571 86
人参皂苷Rb1与相邻组分(ginsenosideRb1andclosetogethercomponent)
1 811 592 07
理论板数(按人参皂苷Rg1峰计)(numberofthetheoreticalplate,calculatedwithreferencetothepeakofginsenosideRg1)
72214100469988
表2 红参样品含量测定结果
Tab2 DeterminationresultofRadixetRhizomaGinsengRubrasamples
含量(content)/%
方法(method)
Rg1+Re
LotNo.0806
HPLC
DiamonsilC18MerckC18
RRLC
AgilentZorbaxEclipse
XDB-C18
0 410 410 41
LotNo.0807
0 590 580 56
LotNo.0806
0 450 440 46
Rb1
LotNo.0807
0 570 610 62
分析时间(analysistime)
/min1203125
溶剂用量(volumeofsolventconsumption)/mL
1203125
19%A 20%A;44~60min,20%A 40%A;60~90min,40%A 55%A;90~100min,55%A 60%A),流
速1 0mL min,检测波长203nm,柱温40#
-1[1][1]
,
进样量10 L。条件二:采用MerckC18(100mm