clontech试剂盒 SMARTer_RACE 5'/3' Kit User Manual
Figure 1. Mechanism of SMARTer cDNA synthesis ............................................................................................................. 4
Figure 2. Overview of the SMARTer RACE procedure.. ....................................................................................................... 6
Figure 3. The relationship of gene-specific primers to the cDNA template. .......................................................................... 9
Figure 4. 5'- and 3'-RACE sample results ............................................................................................................................. 22
Figure 5. Detailed mechanism of the 5'-RACE reactions. .................................................................................................... 27
Figure 6. Detailed mechanism of the 3'-RACE reactions. .................................................................................................... 28 Table of Tables
Table 1. Additional 5'-RACE Sequence Obtained with SMART Technology ....................................................................... 5
Table 2. Setting up 5'- and 3'-RACE PCR Reactions ........................................................................................................... 15
Table 3. Troubleshooting Guide: Other Specific Problems .................................................................................................. 25