clontech试剂盒 SMARTer_RACE 5'/3' Kit User Manual
Stellar Competent Cells (Cat. No. 636763)
Store Stellar Competent Cells at –70°C. Store all other components at –20°C.
10 tubes Stellar Competent Cells (100 µl/tube)
10 tubes SOC Medium (1 ml/tube)
10 µl pUC19 Vector (0.1 ng/µl)
III. Additional Materials Required
If your RNA template is from a non-eukaryotic organism and lacks a polyadenylated tail, you can add one prior to first-strand 3’-cDNA synthesis using the following enzyme:
Poly(A) Polymerase (Takara Bio Cat. No. 2180A)
The following materials are required for In-Fusion cloning and transformation, but not supplied:
Ampicillin (100 mg/ml stock) or other antibiotic required for plating the In-Fusion reaction
LB (Luria-Bertani) medium (pH 7.0)
LB/antibiotic plates
SOC medium
The following material is required for the NucleoSpin Gel and PCR Clean-Up Kit, but not supplied:
96–100% ethanol