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生物化学与生物物理进展Prog.Biochem.Biophys.2015;42(4)
AdvancesinIsolatingCirculatingTumorCellsWithMicrofluidicChips*
L譈Xiao-Qing1),LILei2)**,CHENHong-Mei3),CHENPeng4)**,LIUJing2)
(1)ResearchCenterofBasicMedicalScience,TianjinMedicalUniversity,Tianjin300070,China;
2)
KeyLaboratoryofCryogenics,TechnicalInstituteofPhysicsandChemistry,ChineseAcademyofSciences&
BeijingKeyLaboratoryofCryo-BiomedicalEngineering,Beijing100190,China;
3)InstituteofSemiconductors,ChineseAcademyofSciences,Beijing100083,China;4)CenterforLungCancerDiagnosisandTreatment,TianjinMedicalUniversityCancerInstituteandHospital,Tianjin300060,China)
Circulatingtumorcells(CTCs)areshedbyprimaryormetastatictumors,undergotheAbstractepithelial-mesenchymaltransition,andenterintotheperipheralbloodofpatientswithmetastaticcancers.Itscriticalrolesincancerresearchandclinicaldiagnosticsaregraduallybeingrecognized.ThepresenceofCTCs,especiallythenumberinperipheralbloodcannotonlybeusedforearlydiagnosisofcancerbutalsobeusedtoassessprognosis,andmonitortumormetastasisandrecurrence.Microfluidicchipasahigh-throughput,miniaturizedcellexperimentalplatformhasbeenusedinCTCssorting.Inthisreview,wehighlightrecentprogressesofCTCscapturingofmicrofluidicchipsystemandfocusonthecapturetheory,chipstructureandcaptureefficiencyofvarioustypesofchips.Atlast,weprospectfortheapplicationforegroundofmicrofluidicchiptechnologyinCTCsisolation.Keywordsmicrofluidicchip,circulatingtumorcells(CTCs),tumormetastasis,cellsortingDOI:10.16476/j.pibb.2014.0309*ThisworkwassupportedbygrantsfromKeyResearchProgramoftheChineseAcademyofSciences(KJZD-EW-TZ-L03-6),NaturalScienceFoundationofTianjin(13JCYBJC23600),TheNationalNaturalScienceFoundationofChina(61078074)andChinaPostdoctoralScienceFoundation(2014M550794).**Correspondingauthor.LILei.Tel:86-10-82543766,E-mail:lileilei@mail.ipc.ac.cnCHENPeng.Tel:86-22-23340123,E-mail:chenpengdoc@sina.comReceived:February2,2015
Accepted:March5,2015