核酸序列的基本分析
核酸序列的基本分析结果如下：
SEQ New: 1483 bp;
Composition 388 A; 358 C; 351 G; 386 T; 0 OTHER
Percentage: 26.2% A; 24.1% C; 23.7% G; 26.0% T; 0.0%OTHER Molecular Weight (kDa): ssDNA: 457.73 dsDNA: 914.24 ORIGIN
1 ACGCGGGGGA AAGATGCATC TGCTCGCTTT TCTGTCTCTG AGTACATTCC TGTGCTCTGC 61 GTTCGCTGCT GTTCCTGAGT GGGGCCAATG TGGCGGCATT GGATGGACAG GACAGACCAC 121 TTGCGTTAGT GGTACAGTAT GCGCAGCTCT CAATGACTAT TATTCTCAAT GTGTGCCTGG 181 AACGGCCACA ACAACGGCCG CTCCCACGAC TGCTACATCA ACAACCATTT CTTCCACTTC 241 TCGCACAACT GCTACGTCGA CCACAGCTTC CGCACCATCT TCTACTGGCT TTGTAACTAC 301 CTCTGGCACA GAGTTCCGCC TCAACGGTGC CAAATTTACT ATCTTCGGCG CCAACTCATA 361 CTGGGTCGGG TTGATGGGCT ATAGCACTAC AGATATGAAT AAAGCCTTCG CAGACATCGC 421 GGCTACAGGT GCCACCGTCG TCCGCACATG GGGCTTCAAT GAGGTAACGA GTCCTAACGG 481 GATTTATTAC CAGAGTTGGT CCGGAAGTAC ACCAACTATC AACACAGGTT CTACGGGTCT 541 TCAAAACTTT GATGCCGTCG TCGCTGCTGC TGCTGCACAT GGCTTGAGGC TTATTGTTGC 601 CATAACGAAC AACTGGTCCG ACTATGGTGG AATGGATGTA TACGTTAACC AAATTGTCGG 661 GTCTGGCTCT GCGCACGATT TATTCTATAC CGACTGTGAG GTTATATCTA CTTACATGAA 721 CTACGTCAAG ACCTTCGTCT CGCGCTATGT GAACGAACCT ACTATTTTAG GTTGGGAGCT 781 TGCAAATGAA CCTAGATGCA AGGGGAGTAC CGGGACGACC TCTGGATCAT GCACTGCAAC 841 GACTATCACA AAATGGGCCG CGGCAATTTC AGCGTACATC AAGTCGATCG ATCCCAACCA 901 TCTTGTCGGG ATAGGAGATG AAGGGTTCTA CAATGAACCT AGCGCACCAA CATATCCATA 961 TCAAGGTAGC GAAGGTATCG ATTTTGATGC AAATTTGGCC ATTAGTAGCA TTGATTTCGG 1021 TACATTCCAT TCCTATCCTA TCAGCTGGGG TCAAACCACT GATCCTCAGG GATGGGGTAC 1081 GCAATGGATC GCTGATCATG CAACGTCAAT GACAGCTGCG GGAAAGCCCG TAATCTTAGA 1141 GGAGTTTGGA GTCACCACTA ATCAAGCAAC TGTTTATGGC GCCTGGTATC AGGAAGTTGT 1201 CTCTTCGGGT CTTACTGGTG CTCTTATTTG GCAAGCTGGT TCTTATTTAT CATCCGGAGC 1261 TACTCCGGAC GACGGATATG CAATTTATCC TGATGATCCT GTATATTCCC TGGAAACCTC 1321 CTATGCGGTT ACATTGAAAG CGCGGGCGTA GGATAGGGTA CAGAATAAAT TTTGCTCCGA 1381 TGTGGTACTG TAGCCGAGCG GCTTGACTAT GTGAATAAAA ATAGCACTGT TGTCACGATC 1441 GATCAACACC TAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAA
对其所做对其所做的酶切谱分析结果如下： ① 对DQ286392的酶切图（见附录1） ② 单酶切统计，见下表：
Restriction table:
Enzyme Recognition frequency Positions
__________________________________________________________________________ AccI GT'mk_AC 2 258, 640 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' 1 632 AloI GGAnnnnnnGTTCnnnnnnn_nnnnn' 1 600
AlwI GGATCnnnn'n_ 5 833, 885, 1056, 1095, 1290 ApoI r'AATT_y 3 333, 992, 1368 BanI G'GyrC_C 4 327, 348, 429, 1179 BbeI G_GCGC'C 2 352, 1183 BbsI GAAGACnn'nnnn_ 1 531
BbvI GCAGCnnnnnnnn'nnnn_ 7 53, BceAI ACGGCnnnnnnnnnnnn'nn_ 3 199, 211, 540 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' 3 1003, 998, 1294 BcgI GCAnnnnnnTCGnnnnnnnnnn_nn' 3 969, 1032, 1260 BclI T'GATC_A 1 1094 BfrBI ATG'CAT 1 17 BglI GCCn_nnn'nGGC 1 91 BmrI ACTGGGnnnn_n' 1 371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' 1 605 BsaHI Gr'CG_yC 2 349, 1180 BsaJI C'CnnG_G 2 859, 1309 BsaWI w'CCGG_w 3 501, 1254, 1265 BsaXI ACnnnnnCTCCnnnnnnn_nnn' 1 215 BsaXI GGAGnnnnnGTnnnnnnnnn_nnn' 1 185
BseMII CTCAGnnnnnnnn_nn' 3 30, 67, 1080 BseRI GAGGAGnnnnnnnn_nn' 1 1155 BseYI C'CCAG_C 1 1045 BsgI GTGCAGnnnnnnnnnnnnnn_nn' 1 559
BsiEI CG_ry'CG 3 199, 889, 1440 BsiHKAI G_wGCw'C 2 57, 1223
BslI CCnn_nnn'nnGG 4 81, 449, 963, 1272 BsmAI GTCTCn'nnnn_ 3 40, 743, 1205 BsmBI CGTCTCn'nnnn_ 1 743 BsmFI GGGACnnnnnnnnnn'nnnn_ 1 827 Bsp1286I G_dGCh'C 2 57, 1223 BspCNI CTCAGnnnnnnn_nn' 3 31, 68, 1079 BspEI T'CCGG_A 3 501, 1254, 1265 BsrI ACTG_Gn' 4 290, 366, 618, 1220 BsrBI CCG'CTC 2 201, 1399 BsrDI GCAATG_nn' 1 1089
BstF5I GGATG_nn' 4 108, 641, 1077, 1251 BstZ17I GTA'TAC 1 641
156, 551, 554, 557, 560, 1103 Bsu36I CC'TnA_GG 1 1066 BtgI C'CryG_G 1 859 BtsI GCAGTG_nn' 1 832
Cac8I GCn'nGC 4 25, 781, 1234, 1345 ClaI AT'CG_AT 3 889, 979, 1440 EaeI y'GGCC_r 3 184, 196, 997 EagI C'GGCC_G 1 196 EarI CTCTTCn'nnn_ 1 1208 EciI GGCGGAnnnnnnnnn_nn' 1 306 FauI CCCGCnnnn'nn_ 2 1112, 1336
FokI GGATGnnnnnnnnn'nnnn_ 4 115, 648, 1084, 1238 FspI TGC'GCA 2 143, 673 HaeII r_GCGC'y 2 352, 1183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' 3 690, 1079, 1111 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn' 3 722, 1079, 1111 HincII GTy'rAC 2 259, 647 HpaI GTT'AAC 1 647 HphI GGTGAnnnnnnn_n' 1 1145
Hpy8I GTn'nAC 5 259, 510, 641, 647, 752 Hpy188III TC'nn_GA 10 75, 502, 728, 823, 908, 1191 1255, 1266, 1290, 1435
HpyF10VI GCn_nnnnn'nGC 11 67, 92, 418, 430, 452, 562, 571 574, 871, 997, 1099 KasI G'GCGC_C 2 348, 1179
MboII GAAGAnnnnnnn_n' 5 223, 271, 335, 531, 1195 MlyI GAGTCnnnnn' 2 479, 1159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 1 643
MnlI CCTCnnnnnn_n' 9 311, 1133, 1328 MscI TGG'CCA 1 999 MslI CAynn'nnrTG 1 50
MspA1I CmG'CkG 3 861, 1045, 1116
MwoI GCnn_nnn'nnGC 11 66, 91, 417, 429, 451, 561, 570 573, 870, 996, 1098 NarI GG'CG_CC 2 349, 1180
NlaIV GGn'nCC 5 84, 329, 350, 431, 1181 NsiI A_TGCA'T 1 19
PleI GAGTCnnnn'n_ 2 478, 1158 PshAI GACnn'nnGTC 1 735 PvuI CG_AT'CG 2 889, 1440 PvuII CAG'CTG 2 1045, 1116 SacII CC_GC'GG 1 862 SalI G'TCGA_C 1 257
SfaNI GCATCnnnnn'nnnn_ 5 4, 26, 542, 786, 977
330, 455, 580, 692, 830, 1075 SfcI C'TryA_G 4 380, 388, 424, 1389 SfoI GGC'GCC 2 350, 1181 SmlI C'TyrA_G 1 584 TatI w'GTAC_w 2 42, 507
TspDTI ATGAAnnnnnnnnn_nn' 5 411, 732, 802, 934, 949 TspGWI ACGGAnnnnnnnnn_nn' 1 1288
TspRI _nnCAsTGnn' 3 839, 1064, 1432
Enzymes that cut five or fewer times
Enzyme Recognition frequency Positions
__________________________________________________________________________ AccI GT'mk_AC 2 258, 640 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' 1 632 AloI GGAnnnnnnGTTCnnnnnnn_nnnnn' 1 600
AlwI GGATCnnnn'n_ 5 833, 885, 1056, 1095, 1290 ApoI r'AATT_y 3 333, 992, 1368 BanI G'GyrC_C 4 327, 348, 429, 1179 BbeI G_GCGC'C 2 352, 1183 BbsI GAAGACnn'nnnn_ 1 531
BceAI ACGGCnnnnnnnnnnnn'nn_ 3 199, 211, 540 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' 3 1003, 998, 1294 BcgI GCAnnnnnnTCGnnnnnnnnnn_nn' 3 969, 1032, 1260 BclI T'GATC_A 1 1094 BfrBI ATG'CAT 1 17 BglI GCCn_nnn'nGGC 1 91 BmrI ACTGGGnnnn_n' 1 371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' 1 605 BsaHI Gr'CG_yC 2 349, 1180 BsaJI C'CnnG_G 2 859, 1309 BsaWI w'CCGG_w 3 501, 1254, 1265 BsaXI ACnnnnnCTCCnnnnnnn_nnn' 1 215 BsaXI GGAGnnnnnGTnnnnnnnnn_nnn' 1 185
BseMII CTCAGnnnnnnnn_nn' 3 30, 67, 1080 BseRI GAGGAGnnnnnnnn_nn' 1 1155 BseYI C'CCAG_C 1 1045 BsgI GTGCAGnnnnnnnnnnnnnn_nn' 1 559
BsiEI CG_ry'CG 3 199, 889, 1440 BsiHKAI G_wGCw'C 2 57, 1223
BslI CCnn_nnn'nnGG 4 81, 449, 963, 1272 BsmAI GTCTCn'nnnn_ 3 40, 743, 1205 BsmBI CGTCTCn'nnnn_ 1 743 BsmFI GGGACnnnnnnnnnn'nnnn_ 1 827
Bsp1286I G_dGCh'C 2 57, 1223 BspCNI CTCAGnnnnnnn_nn' 3 31, 68, 1079 BspEI T'CCGG_A 3 501, 1254, 1265 BsrI ACTG_Gn' 4 290, 366, 618, 1220 BsrBI CCG'CTC 2 201, 1399 BsrDI GCAATG_nn' 1 1089
BstF5I GGATG_nn' 4 108, 641, 1077, 1251 BstZ17I GTA'TAC 1 641 Bsu36I CC'TnA_GG 1 1066 BtgI C'CryG_G 1 859 BtsI GCAGTG_nn' 1 832
Cac8I GCn'nGC 4 25, 781, 1234, 1345 ClaI AT'CG_AT 3 889, 979, 1440 EaeI y'GGCC_r 3 184, 196, 997 EagI C'GGCC_G 1 196 EarI CTCTTCn'nnn_ 1 1208 EciI GGCGGAnnnnnnnnn_nn' 1 306 FauI CCCGCnnnn'nn_ 2 1112, 1336
FokI GGATGnnnnnnnnn'nnnn_ 4 115, 648, 1084, 1238 FspI TGC'GCA 2 143, 673 HaeII r_GCGC'y 2 352, 1183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' 3 690, 1079, 1111 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn' 3 722, 1079, 1111 HincII GTy'rAC 2 259, 647 HpaI GTT'AAC 1 647 HphI GGTGAnnnnnnn_n' 1 1145
Hpy8I GTn'nAC 5 259, 510, 641, 647, 752 KasI G'GCGC_C 2 348, 1179
MboII GAAGAnnnnnnn_n' 5 223, 271, 335, 531, 1195 MlyI GAGTCnnnnn' 2 479, 1159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 1 643 MscI TGG'CCA 1 999 MslI CAynn'nnrTG 1 50
MspA1I CmG'CkG 3 861, 1045, 1116 NarI GG'CG_CC 2 349, 1180
NlaIV GGn'nCC 5 84, 329, 350, 431, 1181 NsiI A_TGCA'T 1 19
PleI GAGTCnnnn'n_ 2 478, 1158 PshAI GACnn'nnGTC 1 735 PvuI CG_AT'CG 2 889, 1440 PvuII CAG'CTG 2 1045, 1116 SacII CC_GC'GG 1 862 SalI G'TCGA_C 1 257
SfaNI GCATCnnnnn'nnnn_ 5 4, 26, 542, 786, 977