峰值附近为疏水性较强的序列，即有可能是膜内蛋白部分。
3
首先blast出相应序列，进入
http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastp&BLAST_PROGRAMS=deltaBlast&PAGE_TYPE=BlastSearch&LINK_LOC=BlastHomeAd，点击protein blast，即blastp，输入任意你想blast的序列，记得加标识符>，（fasta兼容），下载mega，安装，打开，我的是
4
在地址栏填入http://swissmodel.expasy.org/interactive，跳转到如下：
注册，在PDB数据库中随便选择一个蛋白质序列，题意是免费平台，所以我们选择p05130先选择search for templates，即选择模板，跳转如下：
? ? ? ? ? ? ? ? ? SWISS-MODEL
Modelling Tools Repository Documentation Log in Create Account All Projects
KPC1_DROME P05130 Protein kinase C
Created: today at 12:05
? ? ?
Summary Templates 0 Models 0 ?
Template Results
The search for templates matching your target sequence is currently running. Please wait.
...reusing cached query alignment
...predicting residue burial status with ACCpro
If you want to come back later, bookmark this link:
http://swissmodel.expasy.org/interactive/7e7Chr/templates/
MSEGSDNNGDPQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGF
GKQGFQC
QVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKCSACDMNVHARCKENVPSLCG
CDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYD
LKPEDKD
RRILIEVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSDTNTHTSS
KKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAI
KILKKDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHS
CFQTMDRLF
FVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFF
LHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFC
GTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQP
NKRLGCG
SSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPEYVFSP
? ? ? ? ? Swiss Institute of Bioinformatics Schwede Group Contact Us Terms of use Back to the Top 正在搜索，结束后如下：
? ? ? ? ? ? ? ? ? SWISS-MODEL Modelling Tools Repository Documentation Log in Create Account All Projects
KPC1_DROME P05130 Protein kinase C
Created: today at 12:05
? ? ?
Summary Templates 50 Models 0 ?
Template Results
? ? ? ? Templates Sequence Similarity
Alignment of Selected Templates More Name
Title
Coverage Identity Method
Oligo State
3pfq.1.AProtein kinase C beta type 67.29 X-ray, 4.0? monomer 3pfq.1.AProtein kinase C beta type 66.87 X-ray, 4.0? monomer 3iw4.2.AProtein kinase C alpha type 73.33 X-ray, 2.8? monomer 3iw4.1.AProtein kinase C alpha type 73.33 X-ray, 2.8? monomer
3iw4.2.AProtein kinase C alpha type 75.30 X-ray, 2.8? monomer 3iw4.1.AProtein kinase C alpha type 75.30 X-ray, 2.8? monomer
2i0e.1.AProtein Kinase C-beta II 71.51 X-ray, 2.6? monomer 2i0e.1.AProtein Kinase C-beta II 72.07 X-ray, 2.6? monomer
1xjd.1.AProtein kinase C, theta type 58.16 X-ray, 2.0? monomer