荧光探针的合成及自由基检测研究 3.3 1H NMR数据 3.3.1 2-(2-吡啶)-苯并噻唑
图3-7 2-(2-吡啶)-苯并噻唑啉
如图4-1所示，1H NMR (400MHz, DMSO-d6):8.53(d, J=4, 1H, pyridine-H), 7.84(t, J=4, 1H, pyridine-H), 7.55(d, J=8, 1H, pyridine-H), 7.33(d, J=8, 1H, pyridine-H), 7.11(s, 1H, C-H), 6.99(d, j=8, 1H, benzothiazole-H), 6.90(t, J=8, 1H, benzothiazole-H), 6.67(d, J=8, 1H, benzothiazole-H), 6.61(t, J=8, 1H, benzothiazole-H), 6.39(d, J=4, 1H, N-H).
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荧光探针的合成及自由基检测研究
3.3.2 2-(4-二甲氨基苯)-苯并噻唑
图3-8 2-(4-二甲氨基苯)-苯并噻唑
如图4-2所示，1H NMR(400MHz CDCl3):7.98(t, J=8, 3H, 2×benzene-H&benzothiazole-H), 7.84(d, 1H, J=8，benzothiazole-H) 7.46(t, J=8, 1H, benzothiazole-H), 7.31(t, J=8, 1H, benzothiazole-H), 6.75(d, J=8, 2H, 2×benzene-H), 3.06,(s, 6H, 2×CH3).
由图4-3可看出两种产物约1:1混合，未氧化探针N原子未形成共轭双键，N-H化学位移为6.39。
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荧光探针的合成及自由基检测研究
图3-9 2-(4-二甲氨基苯)-苯并噻唑及未氧化探针
3.3.3 2-(4-氰基苯)-苯并噻唑
图3-10 2-(4-氰基苯)-苯并噻唑
如图4-4所示，1H NMR(400MHz CDCl3):8.24(d, J=8, 2H, 2×benzene-H),
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荧光探针的合成及自由基检测研究
8.15(d, 1H, J=8, benzothiazole-H), 7.97(d, J=8, 1H, benzothiazole-H), 7.82(d, J=12, 2×benzene-H), 7.58(t, J=8, 1H, benzothiazole-H), 7.48(t, J=8, 1H, benzothiazole-H). 3.3.4 2-(苯)-苯并噻唑
图3-11 2-(苯)-苯并噻唑
如图4-5所示，1H NMR(400MHz CDCl3):8.15(d, J=8, 3H, benzothiazole –H&2×benzene-H), 7.94(d, J=8, 1H, benzothiazole -H), 7.54(s, 4H, 2× benzothiazole –H&2benzene-H), 7.43(t, 1H, J=8, benzene-H).
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荧光探针的合成及自由基检测研究
3.3.5 2-(4-甲基苯)-苯并噻唑
图3-12 2-(4-甲基苯)-苯并噻唑
如图4-6所示，1H NMR(400MHz CDCl3):8.07(d, J=8, 1H, benzothiazole-H), 7.99(d, J=8, 2H, benzothizole-H&benzene-H), 7.89(d, J=8, 1H, benzene-H), 7.50(t, J=4, 1H, benzothiazole-H), 7.37(t, J=4, 1H, benzothizole-H), 7.30(d, J=8, 2H, 2×benzene-H), 2.43(s, 3H, CH3). 3.3.6 2-(水杨醛)-苯并噻唑
如图4-7所示，1H NMR(400MHz CDCl3):8.03(d, J=8, 1H, benzothiazole-H), 7.94(d, J=8, 1H, benzothiazole-H), 7.74(d, J=8, 1H, benzene-H), 7.54(t, J=8, 1H, benzothiazole-H), 7.39~7.46(m, 2H, benzothiazole-H&benzene-H), 7.14(d, J=8, 1H, benzene-H), 6.99(t, J=8, 1H, benzene-H), 1.28(s, 1H, O-H).
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