大孔树脂柱色谱法分离纯化茶皂素的方法研究
大孔树脂柱色谱法分离纯化茶皂素的方法研究
作 者 指导老师 石红于 刘传芳
摘要：:通过吸附、解吸试验，筛选适合分离纯化茶皂素的大孔吸附树脂并确立纯化工艺参数。结果表明，HPD—100型大孔吸附树脂对茶皂素分离效果良好，较佳静态态吸附pH＝3.5，在规格为600mm×40mm分离柱中，装样柱高405mm、洗脱液乙醇浓度为80%、洗脱流速为10 mL/min条件下，洗脱效果最好，在此条件下所得茶皂素回收率为71.70%，纯度达到97%左右。
关键词：油茶籽皂素，提取，分离纯化，大孔吸附树脂;
Macroporous resin column chromatography separation and
purification method of tea saponin
Abstract: : The adsorption, desorption tests, screening for isolation and purification of tea saponin and macroreticular resin purification process parameters established. The results show that, HPD-100 macroporous resin type for separation of the effect of tea saponin good, better static state adsorption pH = 3.5, in the specifications for 600mm × 40mm column, the column-like high-mounted 405mm, ethanol concentration of eluate 80%, elution flow rate of 10 mL / min under the conditions of elution best conditions in the tea saponin recovery from 71.70% for the purity of around 97%
Key words: Oil camellia saponin, extraction, separation and purification, macroporous resin.
1 前言
茶皂素是一种天然表面活性剂[1]，存在于在茶叶、茶籽、和油茶籽中，尤以油茶籽中含量最大。纯茶皂素是白色微细柱状结晶体，分子式为C57H90O26，分子量1203，熔点223-224℃，pH=6.2。分子结构式如下[2]：