本实验对人BMP?9全长读码框架进行了完整克隆，获得了准确且纯度很高的重组载体，为这一多能细胞因子在创伤修复、内分泌、神经系统和造血系统等临床领域的转基因应用实验研究提供了坚实的基础。
【参考文献】
[1]WOZNEY J M, ROSEN V, CELESTE A J, et al. Novel re?gulators of bone formation: molecular clones and activities[J]. Science, 1988,242:1528?1534.
[2]张凌,杨刚毅. 骨形态发生蛋白?9临床研究进展[J]. 医学分子生物学杂志, 2004,1(2):119?121.
[3]SONG J J, CELESTE A J, KONG F M, et al. Bone morphorgenetic protein?9 binds to liver cells and stimulates proliferation[J]. Endocrinology, 1995,136:4293?4297.
[4]MILLER A F, HARVEY S, THIES R S, et al. Bone morphogenetic protein?9 an autocrine/paracrine cytokine in the li?ver[J]. J Biol Chem, 2000,275(24):17937?17945.
[5]LOPEZ?COVIELLA I, BERSE B, THIES R S, et al. Upre?gulating of acetylcholine synthesis by bone morphogenetic protein 9 in a murine septal cell line[J]. J Physiol Paris, 2002,96(1):53?59.
[6]LOPEZ?COVIELLA I, BERSE B, KRAUSS R, et al. Induction and maintenance of the neuronal cholinergic phonotype in the central nervous system by BMP?9[J]. Science, 2000,289(54):313?316.