此外，还可以用胰酶消化组织切片或用10%牛血清蛋白封闭法等消除非特异性染色，提高特异性染色。 票数 + 收藏 +七、现状与展望
免疫荧光组织化学技术经过半个多世纪的不断改进和创新，已成为现代研究生物和医学的重要手段之一。由于免疫荧光技术与形态、机能相结合不断完善和发展，尤其是合成了多种新荧光素与抗体容易结合，结合物稳定。可以和FITC结合进行免疫荧光组织化学双标记或三标记。至今，它已和亲合化学技术如SPA，Biotin及avidin, ConA相结合，应用领域也日益扩大，又与现代的电子计算机和扫描电视技术，共聚焦显微镜，荧光激活细胞分类器（FACS）以及数码相机摄影技术的应用，使得快速性、简便性有了更大的提高，使得定量更加准确。90年代又开展了荧光原位末端标记和荧光原位杂交技术，使得范围更广大。
虽然免疫组织化学有了很大的发展，如免疫金银法，免疫胶体铁法，SP，Evision和CSA法，但由于它有自己独特的优点，特异性强，定位准确、简便、快速、鲜明，在许多领域中仍占有不可取代的地位。如肾活检、皮肤活检中IgG, IgM, IgA, C3等检测，自身抗体的检查，传染病的快速诊断，单克隆抗体的筛选及鉴定等。随着科学技术的不断发展，免疫荧光组织化学技术广泛应用于生命科学的各个领域，放射出更加灿烂五彩缤纷的荧光。
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