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山东农业大学学士学位论文

>CH063_01300长度为75nt，>GLRG_08786长度为76nt。二者的一致性为85%。

内含子⑤：>Seq1 (69 nucleotides)

>Seq2 (71 nucleotides)

16-69 (18-71) 85%

16 CCCCCAGTGTGCAGATAAACCTATTGACGAATACTGACCTTCTCACTTAACCAG 18 CCCCCAGGAAGCAGACAAACCTATTGATGAATACTGACCTTGTCACGTACCCAG ******* ***** *********** ****************** ** ****

>CH063_01300长度为69nt，>GLRG_08786长度为71nt。二者的一致性为85%。 内含子① 内含子② 内含子③ 内含子④ 内含子⑤ 相似度 ch长度 cg长度 77% 204 202 83% 69 59 85% 75 76 82% 62 60 85% 69 71 内含子①的blast结果：

Sequences producing significant alignments:

Select for downloading or viewing reports 1

Description

Max Total Query E Max Accession

score score cover value ident

322 87%

7e-85 99% JF811028.1

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3

4

5

Colletotrichum sp. HZAU RRC XN4-1 beta-tubulin (tub2) gene, 322 partial sequence

Colletotrichum sp. HZAU RRC XN3-1 beta-tubulin (tub2) gene, 322 partial sequence

Colletotrichum sp. HZAU RRC XN3-3 beta-tubulin (tub2) gene, 322 partial sequence

Colletotrichum sp. HZAU RRC XN1-2 beta-tubulin (tub2) gene, 322 partial sequence

Colletotrichum sp. HZAU RRC XN4-5 beta-tubulin (tub2) gene, 316 partial sequence

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322 87% 7e-85 99% JF811026.1

322 87% 7e-85 99% JF811025.1

322 87% 7e-85 99% JF811024.1

316 87% 3e-83 99% JF811027.1

山东农业大学学士学位论文

内含子②的blast结果：

Colletotrichum higginsianum culture-collection 128 100% 4e-27 100% JQ005844.1 IMI:349063 beta-tubulin (TUB2) gene, partial cds 128

Colletotrichum higginsianum culture-collection 128 128 100% 4e-27 100% JQ005845.1 2 MAFF:305635 beta-tubulin (TUB2) gene, partial cds

Colletotrichum tabaci culture-collection CBS:161.53 117 117 100% 8e-24 97% JQ005847.1 3 beta-tubulin (TUB2) gene, partial cds

Colletotrichum fuscum culture-collection CBS:130.57 117 117 100% 8e-24 97% JQ005846.1 4 beta-tubulin (TUB2) gene, partial cds

Colletotrichum sp. HZAU RRC XN4-1 beta-tubulin 117 117 100% 8e-24 97% JF811028.1 5 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN4-5 beta-tubulin 117 117 100% 8e-24 97% JF811027.1 6 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN3-1 beta-tubulin 117 117 100% 8e-24 97% JF811026.1 7 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN3-3 beta-tubulin 117 117 100% 8e-24 97% JF811025.1 8 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN1-2 beta-tubulin 117 117 100% 8e-24 97% JF811024.1 9 (tub2) gene, partial sequence

Colletotrichum destructivum culture-collection 95.3 95.3 100% 4e-17 91% JQ005848.1 10 CBS:149.34 beta-tubulin (TUB2) gene, partial cds

Glomerella truncata culture-collection CBS:127604 89.8 89.8 100% 2e-15 90% JQ005850.1 11 beta-tubulin (TUB2) gene, partial cds

内含子③的blast结果：

1

Colletotrichum fuscum culture-collection CBS:130.57 139 139 100% 2e-30 100% JQ005846.1 beta-tubulin (TUB2) gene, partial cds

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山东农业大学学士学位论文

Colletotrichum higginsianum culture-collection 139 139 100% 2e-30 100% JQ005844.1 IMI:349063 beta-tubulin (TUB2) gene, partial cds

Colletotrichum higginsianum culture-collection 139 139 100% 2e-30 100% JQ005845.1 MAFF:305635 beta-tubulin (TUB2) gene, partial cds

Colletotrichum sp. HZAU RRC XN4-1 beta-tubulin 139 139 100% 2e-30 100% JF811028.1 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN4-5 beta-tubulin 139 139 100% 2e-30 100% JF811027.1 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN3-1 beta-tubulin 139 139 100% 2e-30 100% JF811026.1 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN3-3 beta-tubulin 139 139 100% 2e-30 100% JF811025.1 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN1-2 beta-tubulin 139 139 100% 2e-30 100% JF811024.1 (tub2) gene, partial sequence

Colletotrichum linicola culture-collection CBS:172.51 134 134 100% 1e-28 99% JQ005849.1 beta-tubulin (TUB2) gene, partial cds

Colletotrichum tabaci culture-collection CBS:161.53 134 134 100% 1e-28 99% JQ005847.1 beta-tubulin (TUB2) gene, partial cds

Glomerella truncata culture-collection CBS:127604 128 128 100% 4e-27 97% JQ005850.1 beta-tubulin (TUB2) gene, partial cds

2

3

4567891011

内含子④的blast结果：

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Colletotrichum fuscum culture-collection CBS:130.57 115 115 100% 2e-23 100% JQ005846.1 beta-tubulin (TUB2) gene, partial cds

Colletotrichum higginsianum culture-collection 115 115 100% 2e-23 100% JQ005844.1 IMI:349063 beta-tubulin (TUB2) gene, partial cds

Colletotrichum higginsianum culture-collection 115 115 100% 2e-23 100% JQ005845.1 MAFF:305635 beta-tubulin (TUB2) gene, partial cds

Colletotrichum sp. HZAU RRC XN4-1 beta-tubulin 115 115 100% 2e-23 100% JF811028.1 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN4-5 beta-tubulin 115 115 100% 2e-23 100% JF811027.1 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN3-1 beta-tubulin 115 115 100% 2e-23 100% JF811026.1 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN3-3 beta-tubulin 115 115 100% 2e-23 100% JF811025.1 (tub2) gene, partial sequence

Colletotrichum sp. HZAU RRC XN1-2 beta-tubulin 115 115 100% 2e-23 100% JF811024.1 (tub2) gene, partial sequence

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3

45678

山东农业大学学士学位论文

Colletotrichum destructivum culture-collection 110 110 100% 1e-21 98% JQ005848.1 CBS:149.34 beta-tubulin (TUB2) gene, partial cds

Colletotrichum tabaci culture-collection CBS:161.53 110 110 100% 1e-21 98% JQ005847.1 beta-tubulin (TUB2) gene, partial cds

Colletotrichum linicola culture-collection CBS:172.51 102 102 100% 2e-19 97% JQ005849.1 beta-tubulin (TUB2) gene, partial cds

91011

内含子⑤的blast结果：

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Glomerella graminicola strain M5.001 beta-tubulin 132 132 100% 3e-28 100% JQ005852.1 (TUB2) gene, partial cds

Glomerella graminicola strain M1.001 beta-tubulin 132 132 100% 3e-28 100% JQ005851.1 (TUB2) gene, partial cds

Glomerella graminicola strain DT1062 beta-tubulin 2 132 132 100% 3e-28 100% DQ195741.1 (tub2) gene, exons 2 through 4 and partial cds

132 132 100% 3e-28 100% M34492.1 C.graminicola beta-tubulin-2 (TUB2) gene, complete cds Glomerella graminicola strain DT1024 beta-tubulin 2 126 126 100% 1e-26 99% DQ195747.1 (tub2) gene, exons 2 through 4 and partial cds

Glomerella graminicola strain STE-U 5298 124 124 100% 5e-26 99% AY376587.1 beta-tubulin-2 (tub2) gene, exons 2 through 6 and partial cds

Colletotrichum sublineolum strain BRIP14028 121 121 100% 7e-25 97% DQ195755.1 beta-tubulin 2 (tub2) gene, exons 2 through 4 and partial cds

Glomerella graminicola strain ICMP12090 beta-tubulin 2 (tub2) gene, exons 2 through 4 and partial cds >gb|DQ195745.1| Glomerella graminicola strain 121 121 100% 7e-25 97% DQ195744.1 BRIP14154 beta-tubulin 2 (tub2) gene, exons 2 through 4 and partial cds >gb|DQ195746.1| Glomerella graminicola strain ICMP5171 beta-tubulin 2 (tub2) gene, exons 2 through 4 and partial cds

Glomerella graminicola strain DT378 beta-tubulin 2 121 121 100% 7e-25 97% DQ195743.1 (tub2) gene, exons 2 through 4 and partial cds

Glomerella graminicola strain DT1039 beta-tubulin 2 121 121 100% 7e-25 97% DQ195742.1 (tub2) gene, exons 2 through 4 and partial cds

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910

通过blast比对结果可以看出：

1、 内含子①的长度太长，不利于比较；相似度太低，即变异太大，不适

于做分类的依据。内含子②③④⑤相似度均在80%以上，且长度适宜用于比较，适合作为分类的依据。

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山东农业大学学士学位论文

2、

内含子①作为Query Sequence，能与之匹配的最高为87%，相似度太低，不足以证明其属于哪一亚种，而内含子②③④⑤作为Query Sequence，能与之匹配的最高100%，可以充分证明其属于哪一亚种，作为分类的依据。

3、 内含子②虽然具备作为分类依据的特征，但是我们可以发现它之前的

外显子序列太短，不适合设计引物，这点也使其不适宜作为分类的依据。

综上所述，内含子①②不适合作为分类的依据，内含子③④⑤以其优点可以作为炭疽菌的分类依据。

通过五个基因与系统发育相关的基因在黑粉菌和炭疽菌之间的差异的比较，我们知道黑粉菌没有内含子，炭疽菌有内含子，所以用内含子的变化来作为分类的依据适合炭疽菌、不适合黑粉菌[19]。

更进一步，炭疽菌进化程度低，黑粉菌进化程度高，没有内含子，本文研究的分类相关基因在黑粉菌上不适合，猜测在进化过程中，分类相关基因进化掉了，而黑粉菌是利用别的方式分类的。

通过这些分析数据，可以从功能、结构上获得有价值的信息，为下一步的研究及开发利用做出探索、提出可参考的研究方案[20]。

在人类基因组计划的推动下，以生物信息的采集、处理、存储、传布、分析和解释等多个方面为研究内容的生物信息学得到了很好的发展。本次通过运用生物信息学研究内容的一部分对一个基因序列的结构和功能进行了预测，为下一步研究方案的制定提供了依据，以期研究的顺利进行[21]。

但是，不容忽视的是，目前生物信息还不是一个很完善的学科，所以通过生物信息获取的结论目前并不能直接转化为生产力，用于应用，更多的是作为别的学科的一个佐证，但是在不远的未来，生物信息获取的数据结果一定可以直接产生价值，用于实际的应用

[22]

。

所以，此次论文的写作，更多的是为未来更好的研究提供一种思路，一种方法，开拓新的视野。

参考文献

[1] 朱扬勇，熊赟，上海生物信息技术研究中心，上海，201203

[2] 李伟,茅矛.生物信息学新进展——第六届国际生物信息学和基因组研究年会综

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