Primer Premier 5.0中文使用说明书

2020-02-20 22:54

Primer PREMIER

Version 5.0 for Windows and Power

Macintosh

使用说明书

PREMIER

Biosoft International

3786 Corina way,

Palo Alto, CA 94303-4504

电话 650 856-2703

传真 650 843-1250

电子邮件 sales@PremierBiosoft.com

目

录

目录 ...........................................………………...................................2

新版更新............……………………………………….........................................................6 基础指南.............................................................……………………..................6

GeneTank Window 序列编辑窗口 ………………………………………………7

查看序列..............................................…………………………………….............................7

打开已有序列 ................................................................……………………………….........7 创建新序列...............................................................…………………………………............7 保存序列 .................................................................…………………………………...........8 编辑序列 ..................................................................………………………………..............8 查找.........…………....................................................................................8

查看不同链.....................…………………………...……………..........................................8 语音校读........................…………….....…………….................................................8 编辑序列比较结果.........…………………...............................................................9

GENETANK 序列编辑 ...........................................…................7

序列粘贴窗口..............…………….……………………………............................9 参数设置 .. . . . . . .. . .. . . . . . . . .. . . . . . . … … … … … . . . . . . .. . . . .. . . . . . . . .. . .. .. . .. . . . . . . . .. . . . .. . . . 9 Rating Parameters 评分参数 .......................………………………………….................10

序列比较..................... .......……………………….......................10

Alignment Parameters 序列比较参数设置 ...................………………...........................10

序列翻译...........................………………………………………….....................12

Edit Codon Table 窗口...........………………………………………………………...............12

Primer Premier 引物设计窗口 ........................…….....................................14 Direct Select 即点即选 14

框.....................................................................…..........

在序列比较中应用即点即选功能..........………………………….............................…........14

性状列表 ....................................……………........................................15 二级结构 ........................................................................…………..15

PRIMER 引物设计 .........................…….............................….....14

Edit Primer 引物编辑窗口 .............…..........……..........................................16

Search Criteria 搜索标准窗口 ............……..................................................16

Search Parameter 搜索参数窗口 ....……………………................................17

Automatic Search 自动搜索 ........... …………………....................................................17 Manual Search 手动搜索及引物设计原理......………………………...............................18

Search Results 搜索结果窗口 ...........……………........................................20

Multiplex/Nested Primer 复式及巢式PCR反应引物窗口 ...………………...21

Database 引物数据库窗口 ..............................…….....................................21

...................................Reports 分析报告……....................................22

Synthesis Order Form 合成订单 ................…………...............................22

Optical Activity 比吸光度 ..................................………………....................................22

Degeneracy 多义性………………..................................................23 ..........................

..........................Graphs 图表………...................................................23

Restriction Enzyme Analysis

……..........24 Restriction Enzyme Analysis 限制性内切酶分析窗口 .............

Restriction Sites 酶切位点窗口 ..............................…………..................24

Edit Enzyme 限制内切酶编辑窗口 .....…………………...............................24

Filter 特征筛选窗口 ....................................…………....................................25

限制性内切酶分析 .………....24

Motif Analysis

Motif Analysis 基序分析窗口…………….....................................26 ...............

窗口.....................……......................................26 Motif Sites 基序位点

窗口....................……..........................................26 Edit Motif 基序编辑

基序分析 ......... ……………...........................26

Menu Commands

GeneTank 主菜单.............................. …….........................................27

菜单命令 ...... ………....................................27

File....................................................……………...................................................27

Edit ..................................................…………….................................................27 View...................................................………….................................................27 Search ...............................................…………................................................27 Function............................................…................................................27

Translate ...........................................……………...............................................28 Window......................................………........................................................28 Help .........................................………….....................................................28

Primer 主菜单.................................................................................28

File.....................................................................................................28

E d i t .. . .. . .. . . . . . . . .. .. . . . . .. . .. . .. . . …… … … … … . .. . .. . . . . . . . .. .. . . .. . .. .. . .. . .. . . . .. . .. . .. 2 8 Function.......................………………….....................................................28

Report.................……………................................................................................28 Graph..............…………..................................................................................28

Window...........……………….....................................................................................29 Help ...........………….......................................................................................29

Enzyme Results Menu 主菜单..........……………….....................................29

File...........................…………...........................................................................29 Function..............……………................................................................................29 Window.........................…………….....................................................................29 Help ............................…………........................................................................29

Motif Menu 主菜单..................................................................................29

File...........................……………...........................................................................29 Function..................……………….........................................................................29 Window.....................……………….........................................................................29 Help ........................................………………..........................................................29

附录......................………………............................................30

…………….…………………………………………..........31 附录 A 公式法则

.………………..…………………………......................33 附录 B 多义碱基表

……….….………………………………..................34 附录 C 氨基酸缩写代码

...…………….…………………………….......................35 附录 D 软件上限

.……………….…………………….............................36 附录 E 参考文献

……………….………………………………..................37 附录 F 系统要求

服务与技术支持.............…………………………….............................................38 其他信息...................…………………….....................................................38 译者后记...................……………………….....................................................38

Information in this manual may change without notice and does not represent a commitment on the part of PREMIER Biosoft International.

The software described in this manual is provided by PREMIER Biosoft International under a License Agreement. The software may be used only in accordance with the terms of the agreement.

PREMIER Biosoft International claims copyright to this program and documentation as an unpublished work. Claim of copyright does not imply waiver of Premier's other rights.

This program and documentation are confidential and the property of Premier. Use,

examination, reproduction, copying, decompilation, transfer, and/or disclosure to others are strictly prohibited except by express written agreement with Premier.

软件的使用必须在授权范围之内该软件及相关文档作为一项未公开发表的工作其版权归

Premier所有对版权的声明并非意味放弃其他权利该软件及相关文档是Premier的机密与财产除非有Premier 检查再生产的书面授权任何的使用复制分割传递或 /和解密行为均被禁止

译者声明

本软件说明书著作权归PREMIER Biosoft International 所有译者则保留中

文翻译的相关版权并授权生物软件网 www.bio-soft.net 共享发布该说明书的中文翻译本中文翻译仅限于软件使用者参考其内容如有歧义应以原英文说明书为准译者及生物软件网不承担由翻译打印网页等错误带来的直接和间接损失

译者 Wenkelly

电子邮件 wenkelly163@163.com

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